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June 13th, 2014, 03:49 PM
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1. a) Draw a graph that will represent the following equation:
dA =kA
dt
(5)
b) Describe a method to determine surface area of a leaf without using any
instrument.
(5)
c) Water is often contaminated by a variety of ionic impurities. What is the
best method to determine purity of a sample of water?
(5)
d) You have to test if ponds A, B and C differ significantly in their primary
productivity. What statistical test should be applied to your data? Justify your
choice of the test.
(5)
2. The extensive sequences (Gly-X-Pro)n or (Gly-X-Hpro)n stabilize the collagen
triple-helix, in which X is any amino acid.
(i) Why must Gly be present in every third residue?
(5)
(ii) What are the principal bonds, which are responsible for holding three
helical strands together in the superhelix?
(4)
(iii) How does each strand of the collagen helix differ from an α-helix in terms
of structural characteristics?
(3)
3. (a) An experiment was done to determine the effect of compound Y on the
biochemical reaction A + B → C. It was found that in the presence of Y,
very little, if any, product C was formed. However, on increasing the
concentration of A, C could be detected. Explain the effect of Y on the
reaction.
(2)
(b) Describe an approach by which secondary structure of RNA molecules
can be verified.
(5)
(c) How would you determine the aggregation state and shape of a biomacro- molecule in solution?
(5)
4. a) What are the two tautomeric forms of cytosine? Show with the help of a
diagram.
(6)
b) How could one determine the degree of polymerization of a sample of
amylose?
(6)
5. a) Draw diagrams to illustrate sequence-dependent variation between two
adjacent base pairs, known as tilt, roll and twist.
(6)
b) What is the major difference between normal DNA double helix and RNA
double helix? How does this influence the biological function of these two
molecular forms?
(6)
6. A liver cell has been pulse-labeled with [3H] uridine and a thin section of it is
obtained. The overall appearance of the autoradiograph (examined through
electron microscope) shows regions of white patches interspersed with black dots (silver
grains).
1) What are these sites?
(2)
2) Which site is functionally more active and why?
(4)
3) One of these sites is thought to contain DNA that is never transcribed in
any cell. Name the site and its physiological significance, if any.
(6)
7. (a) The basic function of the cell cycle is to duplicate the DNA accurately and
then distribute the copies precisely to daughter cells. So, why are there
gaps between S phase and M phase?
(4)
(b) What are DNA damage checkpoints which block the cell cycle
progression in G1 and G2 phases?
(8)
8. (a) What is the role of cross-linking glycans, in plant cell wall synthesis?
Name three cross-linking glycans, their structure and composition.
(6)
(b) “Cytoskeletal polymers have an intrinsic polarity”. Comment on the
statement.
(6)
9. a) What are the four major phospholipids present in the plasma membrane of
mammalian cells?
(4)
b) What is the distribution of these phospholipids in the outer and inner
monolayer in human red cell membrane?
(4)
c) The plasma membrane contains various phospholipases that are activated
by extracellular signal to cleave specific phospholipids. What is the
function of phospholipase C in response to extracellular signals?
(4)
10. (a) Draw a diagram of a DNA molecule undergoing replication. Indicate the
newly synthesized strands and identify the following:
(5)
i. Polarity of newly synthesized strands
ii. Leading and lagging strands
iii. Okazaki fragments
iv. RNA primers
(b) Cycloheximide and chloramphenicol both inhibit protein synthesis by
blocking peptidyl transferase. How are these two inhibitors different?
(4)
(c) Name the three enzymes involved in the formation of 5′-cap in
eukaryotic mRNA.
(3)
11. (a) (i) What are the three components, which arise from the 45S rRNA
precursor molecule?
(ii) If 3H labeled uridine is fed to a cell transcribing rRNA genes, in
which rRNA gene product would the label appear first?
(iii) What is the major advantage of getting the three rRNA genes
transcribed together?
(6)
(b) Show pictorially the organization of the lac operon of E. coli.
(3)
(c) Design an experiment to show that induction of host protein synthesis
stops after T4-phage infection.
(3)
12. (a) Mention two approaches used for deciphering the genetic code.
(6)
(b) Insertion of a base in a coding sequence leads to a shift in the
reading frame, which in most cases produces a nonfunctional
protein. What kind of mutation in a tRNA might suppress frame
shifting?
(2)
(c) Bacteriophage T4 rapidly injects the total genome inside the host cell
while T7 injects the genome slowly. Explain the reason.
(4)
13. White blood cells (WBCs) lead a nomadic life, moving between the blood-stream and the
tissues, necessitating the presence of special adhesive properties to bind to the
endothelium in collaboration with integrin.
(a) Name the various molecules responsible for these adhesive properties.
(3)
(b) With the help of some appropriate experiments prove the localization of
these molecules on the cell surface and their carbohydrate-binding
properties.
(5)
(c) What is the nature of collaboration of these molecules with “integrin”?
Why is it crucial for WBC’s life?
(4)
14. (a) Giving one example each, describe the characteristics of autocrine and
paracrine signaling.
(4)
(b) Explain how a single epinephrine molecule can generate numerous Ca+2
ions as second messenger.
(4)
(c) Describe how a cAMP mediated signal can be terminated?
(4)
15. (a) What is the function of an adjuvant in immune response?
(2)
(b) When a virus is taken up by a dendritic cell, how is it processed and
presented to the T cell?
(5)
(c) What are transgenic and knockout mice and how are they developed?
(5)


41. During protein synthesis, L-amino acid binds to t-RNA through
1. α-amino group.
2. hydrophobic side chain.
3. α–carboxyl group.
4. carboxyl group of the side chain.
42. The peptide bond is planar
1. due to restriction caused by rotation around cα–N bond
2. due to restriction around cα–c′ bond
3. due to delocalization of the lone pair of electrons of the nitrogen onto
carbonyl oxygen
4. because amide protons and carbonyl oxygen are involved in hydrogen
bonding.
43. Hydrogen bond length will NOT be
1. independent of the nature of donor and acceptor atoms.
2. dependent on donor and acceptor atoms.
3. dependent on the solvent in which the molecule is dissolved.
4. dependent on the other atoms bonded with the donor and acceptor atom.
44. Why a DNA duplex melts at a specific temperature (Tm) on heating?
1. Loss of base stacking energy
2. The double helix is intrinsically unstable
3. The single helix is more stable as compared to the double helix
4. The DNA double helix is a co-operative structure stabilized by hydrogen
bonds and base pairing
45. Lipid bilayers can be formed by phospholipids which have variable head groups
and fatty acyl chains. The fluidity of the membrane will depend on
1. only the nature of head groups.
2. only the length of the fatty acid chains irrespective of the extent of
unsaturation.
3. only unsaturation irrespective of the length of the fatty acid chains.
4. length and degree of unsaturation of fatty acid chains.
46. Which one of the following RNA molecules is involved in regulation of gene
expression?
1. miRNA
2. rRNA
3. 5S RNA
4. tRNA
47. In which organelle is NADP+ the final electron acceptor?
1. Only chloroplast
2. Only mitochondrion
3. Both chloroplast and mitochondrion
4. Lysosome
48. When the K'q = 1, ∆G° is equal to
1. –1.
2. 0.
3. +1.
4. 10.
49. Which one of the following human pathogen is a flagellated protozoan?
1. Trypanosoma
2. Plasmodium
3. Paramecium
4. Entamoeba
50. Which of the following mammalian cells usually does NOT divide in adult life?
1. Epithelial cells in lung
2. Nerve cells in brain
3. Liver cells
4. Osteoblast cells
51. What happens to the Cdk-cyclin A complex at metaphase?
1. Both cyclin A and Cdk remain undegraded
2. Only Cdk is degraded
3. Only cyclin A is degraded
4. Both cyclin A and Cdk are degraded
52. The average human genome has approximately 3×109 base pairs coding for
various proteins. If an “average” protein contains 400 amino acids, what is the
maximum number of proteins that can be encoded by the human genome?
1. 2.5 × 106.
2. 2.5 × 107 .
3. 3.0 × 106 .
4. 3.5 × 107 .
53. In eukaryotes, the interaction of enhancer and promoter elements is brought
closer by
1. zinc finger.
2. DNA looping.
3. helix turn helix.
4. palindrome.
54. Which of the following cytoskeleton elements guides the movement of vesicles
containing cell wall precursors from their site of formation in Golgi to the site of
new wall formation in a growing pollen tube?
1. Myosin
2. Actin
3. Kinesin
4. Dynein
55. Ionophores are small hydrophobic molecules that can partition into the lipid
bilayer and increase their permeability to specific inorganic ions. Which of the
following is a channel forming ionophore?
1. Valinomycin.
2. Actinomycin.
3. Gramicidin A.
4. Nicin.
56. Which GTPases regulate intracellular transport in mammalian cells through
vesicle fusion?
1. Rab
2. Ran
3. Ras
4. Rho
57. The region where RNA polymerase binds to promoter in prokaryotes is called
1. Pribnow box.
2. Hogness box.
3. Homeo box.
4. Shine-Dalgarno box.
58. Which of the following features highlights the difference between Z-DNA and B- DNA?
1. Double helical nature
2. Orientation of phosphate backbone
3. Pairing of G – C
4. Antiparallel nature of two polynucleotide strands of double helix
59. Ribosomal subunits are assembled in
1. cytoplasm.
2. nucleolus.
3. nucleus.
4. endoplasmic reticulum.
60. The absence of sigma factor in RNA polymerase
1. affects elongation only.
2. blocks initiation only.
3. affects both initiation and elongation.
4. does not affect transcription.
61. Which of the following features is not required in the initiation step of protein
synthesis?
1. Amino acid activation
2. Binding of mRNA to the ribosomes
3. Transfer of activated amino acid to tRNA
4. Joining together of two amino acids by peptide bond formation
62. By which of the following mechanisms does cycloheximide inhibit protein
synthesis?
1. Blocking the peptidyl transferase of 80S euokaryotic ribosomes
2. Blocking the peptidyl transferase of 70S prokaryotic ribosomes
3. By binding to DNA dependent RNA polymerase
4. By binding to sigma factor
63. Which of the following is a natural inducer of the lac operon in E. coli?
1. Lactose
2. Galactose
3. Allolactose
4. IPTG
64. Antitermination of RNA synthesis is a major mechanism of regulation in
1. lytic phase of λ phage.
2. lysogenic phase of λ phage.
3. lac operon.
4. trp operon.
65. Influenza virus binds to its host cells through which one of the following
carbohydrate moiety.
1. N-acetyl glucosamine
2. N-acetyl neuraminic acid
3. Fucose
4. Mellibiose
66. In mammals, G protein coupled receptors (GPCR) play a major role in mediating
effects of various hormones NOT through
1. activation of protein kinase A.
2. activation of adenylate cyclase.
3. inactivation of adenylate cyclase.
4. activation of tyrosine kinase activity.
67. Receptors for neurotransmitters are located on the
1. cell surface.
2. nucleus.
3. endosome.
4. Golgi apparatus.
68. One of the major transmembrane proteins in a “tight junction” is
1. lectin.
2. claudin.
3. adherin.
4. integrin.
69. Retroviruses are well known as cancer causing agents because it
1. causes mutations in host genes involved in growth.
2. integrates their proviral DNA next to protooncogenes.
3. generates defective viruses lacking some of the viral genes.
4. integrates their T antigens into the host genome.
70. Select the correct statement:
1. In vertebrate development, immune and nervous systems are
the prevalent sites for apoptosis.
2. Apoptosis is triggered by growth stimulus.
3. The tumor suppressor factor p53 inhibits apoptosis.
4. The soluble form of tumor necrosis factor cannot induce apoptosis.
71. ELISA assay
1. uses complement mediated cell lysis.
2. uses a radiolabeled second antibody.
3. involves addition of substrate which is converted to coloured end product.
4. requires specialized red blood cells.
72. In mosaic development, the prospective potency of cells
1. equals its prospective fate.
2. is greater than prospective fate.
3. is less than prospective fate.
4. and fate are unrelated.
73. Experiments with sea urchin demonstrated species specific sperm-egg
recognition through the protein
1. bindin.
2. avidin.
3. activin.
4. hyalin.
74. Exposing a regenerating limb to which of the following chemicals results in the
blastema proximalization?
1. Ascorbic acid
2. Thyroxine
3. Retinoic acid
4. Glutamic acid
75. Temperature-dependent sex determination is observed in
1. Drosophila.
2. amphibians.
3. reptiles.
4. sea urchins.
76. Some plants require vernalization (prolonged cold treatment) for transition to
flowering. For floral induction, vernalization signal is perceived primarily by
1. young leaves subtending the apical meristem.
2. mature leaves near the root-shoot junction.
3. all vegetative parts.
4. shoot apical meristem.
77. Which phase of embryogenesis in plants is characterized by the initiation of
deposition of storage reserves?
1. Globular stage
2. Heart stage
3. Torpedo stage
4. Cell enlargement stage
78. Which of the following statements is NOT true in relation to growth of animals?
1. When all body parts grow at the same rate, it is called isometric growth.
2. When different body parts grow at different rates, it is called allometric
growth.
3. Two-fold change in weight will cause a 1.26-fold expansion in length if
growth is allometric.
4. Isometric growth cannot create dramatic changes in the structure of
organisms.
79. Abnormalities during development caused by exogenous agents are called
disruptions and the agents are specifically called
1. morphogen.
2. teratogen.
3. allergen.
4. mutagen.
80. At which of the following steps does application of dichlorophenyl-dimethyl urea
(DCMU) inhibit electron flow during photosynthesis?
1. P680* → Pheophytin
2. QA → QB
3. QB → Cytb6f complex
4. Cytb6f complex → plastocyanin
81. A major functional difference between the succinyl CoA-synthetase of plant and
animal cell mitochondria is that it
1. does not produce ATP in plant cell.
2. does not produce GTP in plant cell.
3. produces ATP in plants and GTP in animals.
4. produces GTP in plants and ATP in animals.
82. A common symptom of molybdenum deficiency in plants is the accumulation of
nitrate in the cytosol, which results from
1. reduced nitrite reductase activity.
2. reduced nitrate reductase activity.
3. reduced transport of nitrate into the vacuoles.
4. reduced transport of nitrate into the chloroplasts.
83. The Rht mutations in wheat that were pivotal for 'Green Revolution' cause
reduction in plant height due to impairment in
1. gibberellic acid biosynthesis pathway.
2. gibberellic acid signaling pathway.
3. auxin biosynthetic pathway.
4. auxin response pathway.
84. Which of the following phytochrome controlled responses displays red/far-red
reversibility?
1. Very low-fluence responses
2. Low-fluence responses
3. High-irradiance responses
4. Very high-irradiance responses
85. At permanent wilting point, plants cannot regain turgor pressure even if
transpiration stops because
1. water potential of soil (ψw) is less than or equal to osmotic potential (ψS)
of the plant.
2. ψw is higher than ψS .
3. ψw and ψS are unaltered.
4. ψS remains unaltered.
86. Alkaloid production in plants is regulated by a change in the endogenous pool of
1. gibberellins.
2. jasmonates.
3. brassinosteroids.
4. abscisic acid.
87. Flooding and submergence of plants leads to anoxic conditions. Usually, flood- tolerant species alter their developmental programme, resulting in
1. increased activity at the apical meristem.
2. growth retardation of foliage.
3. elongation growth of their stems.
4. increased proliferation of root system.
88. An organism with the genotype AABbCcDD is selfed. The four genes are
independently assorting. What proportion of the progeny will show the genotype
AAbbccDD?
1. 1/4
2. 1/16
3. 1/64
4. 1/256
89. Two fruitflies with mutant eye color were crossed. All progenies obtained from
this cross had wild type eye colour as
1. the mutations are allelic.
2. one mutation is dominant over the other.
3. the mutations are co-dominant.
4. the mutations are in two different genes.
90. The following pedigree represents the inheritance of a rare disorder.
Based on the above pedigree, what is the most likely mode of inheritance?
1. Autosomal dominant
2. X-linked recessive
3. X-linked dominant
4. Y-linked dominant
91. Which of the following pair of relatives will have the highest genetic correlation?
1. First double cousins
2. Half siblings
3. Brothers
4. Brother-sister
92. a1, a2, a3 are three alleles of a gene in Neurospora. Crosses between different a
mutants gave the following results
The process which best explains this result is
1. gene conversion.
2. forward mutation.
3. non-homologous recombination.
4. incomplete dominance.
93. You are provided with an E. coli strain auxotrophic for the amino acids Trp, Leu
and Val. To select for double revertants in Trp and Leu, which amino acid(s)
would you include in the growth medium?
1. Trp
2. Leu
3. Val
4. Trp, Leu, Val
94. UAG and UAA are both nonsense codons. What kind of single point mutation
would cause reversion of UAG to a meaningful codon?
1. Transition
2. Transversion
3. Frameshift
4. Inversion
95. E. coli cells were simultaneously infected by two rII bacteriophage mutants.
From the progeny obtained after lysis of the E. coli cells it was observed that
some of the bacteriophages showed a wild type phenotype. These were
obtained at extremely low frequency. This is due to
1. complementation of the two mutations.
2. recombination between the two mutant chromosomes.
3. transposition of the mutation.
4. incomplete penetrance.
a1 × a2

a1 × a3

a2 × a3

a1+ a1+ a2+
++ a1+ a2+
+ a2 ++ ++
+ a2 +a3 + a3
96. Which of the following hormones stimulates the reabsorption of Na+ and the
secretion of K+ in the kidney?
1. Vasopressin
2. Thyroxine
3. Prolactin
4. Aldosterone
97. The correct sequence in vertebrate embryonic development is
1. gastrocoel – blastocoel – notochord – neural crest.
2. blastocoel – gastrocoel – neural crest – notochord.
3. gastrocoel – blastocoel – neural crest – notochord.
4. blastocoel – neural crest – gastrocoel – notochord.
98. A person has a vision problem caused by the image of an object at infinity getting
focused in front of retina. The error can be corrected by the use of
1. biconvex lens.
2. cylindrical lens.
3. plano convex lens.
4. biconcave lens.
99. During electrical stimulation-induced depolarization of neuron, voltage-gated
1. Na+ channels will close.
2. K+ channels will close.
3. Cl− channels will open.
4. Na+ channels will open.
100. The primary function of diaphragm is to
1. control blood pressure.
2. regulate respiration.
3. support the heart.
4. keep the rib cage dilated.
101. In nephron, the main role of deamination is to
1. reduce urine pH.
2. reduce water loss.
3. release urea.
4. release uric acid.
102. During inspiration, the air that we breathe moves through different regions of the
associated organs in the sequence of
1. larynx > nasopharynx > trachea > glottis.
2. nasopharynx > glottis > larynx > trachea.
3. glottis > nasopharynx > larynx > trachea.
4. larynx > glottis > nasopharynx > trachea.
103. Knee jerk reaction is an example of what type of reflex?
1. Monosynaptic
2. Multisynaptic
3. Conditioned
4. Conscious
104. Which of the following processes is a major problem in interpreting molecular
phylogeny?
1. Horizontal transfer of genes
2. Gene duplication
3. Synonymous mutations
4. Non-synonymous mutations
105. In a tissue, a cell that markedly differs in form, size and content from other cells
of the same tissue is called
1. intermediary cell.
2. isotropic cell.
3. idioblast cell.
4. myrosin cell.
106. Among the extant reptiles which group is phylogenetically closely related to
Aves?
1. Turtles
2. Lizards
3. Snakes
4. Crocodiles
107. The group of organisms that is now separated from the other groups of fungi
based on their motile spores and cellulose-rich cell wall is
1. Myxomycetes.
2. Zygomycetes.
3. Deuteromycetes.
4. Oomycetes.
108. Some floristic elements common to both India and China are in the genus
1. Ginkgo.
2. Rhododendron.
3. Poeciloneuron.
4. Erinocarpus.
109. The difference between Indian and African wild herbivore fauna is that there are
no
1. antelopes in India.
2. deer in Africa.
3. odd-toed animals in India.
4. even-toed hoofed animals in Africa.
110. Which of the following bird species is endangered?
1. Hill myna
2. Great Indian bustard
3. Crow-pheasant
4. Grey hornbill
111. In spite of the prevalence of herbivory, the earth continues to be largely green
because
1. the number of herbivore species is low.
2. herbivores are very inefficient feeders.
3. herbivore numbers are kept low by their predators.
4. herbivory promotes plant growth.
112. Which of the following curves represents the general relationship between body
size (S) and intrinsic rate of population growth (r) ?
113. Which of the following reproductive strategies is characteristic of marine
invertebrates?
1. Long generation time, small clutch size
2. Short generation time, small clutch size
3. Long generation time, large clutch size
4. Short generation time, large clutch size
114. Bergmann’s Rule refers to a general tendency of mammals to be
1. larger in size in colder areas of their distribution.
2. smaller in size in areas of their distribution.
3. darker-pigmented in warmer areas of their distribution.
4. lighter-pigmented in warmer areas of their distribution.
115. When removal of a species from an ecosystem affects persistence of many other
species and the impact of that species removal is disproportionate to its
abundance, the species is known as
1 indicator species.
2. keystone species.
3. flagship species.
4. umbrella species.
116. Biomass turnover time is the ratio between biomass and productivity of an
ecosystem. Which of the following forests should have highest biomass turnover
time?
1. Tropical dry forests
2. Tropical wet forests
3. Temperate deciduous forests
4. Boreal forests
117. Conversion of nitrite to nitrate in soil is done by the bacteria of genus
1. Azotobacter.
2. Nitrosomonas.
3. Nitrobacter.
4. Pseudomonas.
118. In a population with two alleles ‘a’ and ‘b’ of a genotype in a ratio of 0.2 and 0.8
in Hardy-Weinberg equilibrium, how many individuals in a sample of 300 can be
expected to be homozygous for allele ‘a’?
1. 192
2. 12
3. 64
4. 96
119. Defective alleles are eliminated rapidly from a population if they are
1. recessive.
2. dominant.
3. codominant.
4. in multiple copies.
120. The correct expression of Hamilton rule for the evaluation of altruism is [C = the
cost of a behavioral act to the actor, b = the benefit of that act to a beneficiary, and
r = the genetic relatedness between the actor and the beneficiary]
1. c < b⋅r
2. c < b
3. c⋅r < b
4. r < b⋅c
121. The evolutionary basis of sexual dimorphism is
1. differential investment in offspring.
2. difference in aggression.
3. difference in sex chromosomes.
4. difference in autosomes.
122. The evolutionary appearance of the first mammals was
1. after the extinction of dinosaurs and before the appearance of birds.
2. before the extinction of dinosaurs and after the appearance of birds.
3. before the extinction of dinosaurs and before the appearance of birds.
4. after the extinction of dinosaurs and after the appearance of birds.
123. Both the Luria-Delbruck experiment and the Lederberg and Lederberg experiment
demonstrate
1. pre-selection mutations.
2. post-selection mutations.
3. directed mutations.
4. adaptive mutations.
124. Vampire bats regurgitate food in order to feed a starving member of their group.
This is an example of
1. group selection.
2. reciprocal altruism.
3. selfish behavior.
4. K-selection.
125. The first appearance of amphibians on earth was during the period
1. Silurian.
2. Carboniferous.
3. Triassic.
4. Jurassic.
126. In a transgenic mouse experiment a founder male produces 100 pups and only 20
of these are transgenic. This result leads to the conclusion that the
1. founder transgenic animal is chimaeric.
2. founder animal is mosaic.
3. transgene is integrated on Y–chromosome.
4. transgene is integrated on X–chromosome.
127. An inbred mouse is cloned using another mouse strain as an egg donor. The
genetic relationship between the original inbred mouse and its clone will be
1. 100%.
2. 99–100%.
3. 95–99%.
4/ 90–95%.
128. Which of the following elements can be used to immobilize a reduced protein?
1. Calcium
2. Potassium
3. Gold
4. Sodium
129. D-amino acids
1. cannot be produced by fermentation.
2. can be produced by E. coli.
3. can be produced by yeast.
4. can be produced by microbes provided with chiral precursors of D-amino
acids.
130. Which of the following viruses is used for biocontrol of insect pests of plants?
1. Cauliflower mosaic virus
2. Cucumber mosaic virus
3. Rice tungro virus
4. Nuclear polyhedrosis virus
131. What ratio do you expect for codominant loci in F2 populations?
1. 1:1
2. 3:1
3. 1:2:1
4. 9:3:3:1
132. The copy number of a transgene in plants can be deciphered by
1. Southern blotting.
2. northern blotting.
3. south western blotting.
4. far western blotting.
133. It is hypothesized that the mean (µ) longevity of a Drosophila strain is 18 days,
with a variance (σ) of 3 days. What values of longevity in a sampled population
will lead to rejection of the null hypothesis at 95% confidence level?
1. Only values less than 15.
2. Values less than 15 and more than 18
3. Only values more than 21
4. Values less than 12 and more than 24
134. Normalized Differential Vegetation Index (NDVI) in remote sensing refers to the
following spectral band derivation:
1. Near IR – Red
2. Red / Near Red
3. (Near IR – Red) / (Near IR + Red)
4. (Near IR – Red) / Red
135. Function of a monochromator in a spectrophotometer is
1. focusing a straight beam of light.
2. dividing a light beam into its component wavelengths.
3. selecting a desired wavelength.
4. creating a light source.
136. Which of the following methods is the most appropriate for estimating the
population density of burrowing animals?
1. Quadrat sampling
2. Line transect sampling
3. Tag-recapture method
4. Nearest neighbour distance method
137. A radioactive sample was counted in a scintillation counter and a value of 194930
cpm was obtained. The counting efficiency was found to be 95.7%. What is the
actual amount of radioactivity present in the sample?
1. 181171 dpm
2. 190411 dpm
3. 203600 dpm
4. 211326 dpm
138. Which one of the following treatments does NOT enhance the response of a film
to radioisotopes in autoradiography?
1. Staining the gel with Coomassie blue before drying
2. Use of intensifying screens
3. Exposure at low temperature
4. Preflashing the film with a light flash
139. What is the length of oligonucleotide required to give consistent specific
hybridization signal in a microarray?
1. 5
2. 7
3. 9
4. 20
140. A protein is poorly expressed in a diseased tissue. To determine whether the
defect is at the level of transcription or translation, which of the following
blotting methods would you use?
1. Southern
2. Southern and northern
3. Northern and western
4. Western


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University Grants Commission New Delhi
Bahadur Shah Zafar Marg,
Balmiki Basti,
Vikram Nagar,
New Delhi,
Delhi 110002 ‎
093 33 778791 ‎
India

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Last edited by Neelurk; March 19th, 2020 at 02:06 PM.
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