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  #1  
July 25th, 2017, 02:50 PM
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DNASE Agar HCL

Hi I would like to know what Deoxyribonuclease (DNase) test is utilized as well as the requirements and the process to be followed?
  #2  
July 25th, 2017, 03:04 PM
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Join Date: Mar 2012
Re: DNASE Agar HCL

DNA Hydrolysis test or Deoxyribonuclease (DNase) test is utilized to decide the capacity of a creature to hydrolyze DNA and use it as a wellspring of carbon and vitality for development.

An agar medium; DNase agar, a differential medium is utilized to test the capacity of a creature to deliver deoxyribonuclease or DNase.

This medium is light green in shading in view of DNA-methyl green (marker) complex (Note: Methyl green is a cation which ties to the adversely charged DNA). It likewise contains supplements for the microbes.

In the event that the life form that develops in the medium produces Deoxyribonuclease, it separates DNA into littler parts. At the point when the DNA is separated, it never again ties to the methyl green, and green shading blurs and the province is encompassed by a dry zone



Necessities:

Media: DNase Agar or DNase agar with Methyl green marker.

Reagent: Hydrochloric corrosive (1mol/L) just when DNase agar without pointer is utilized

Others: Inoculating circle, Bunsen burner

Methodology of DNase (DNA hydrolysis test)

Dry the Surface of agar plates before utilize. Each plate might be isolated into segments by drawing lines on the base of the plate.

Vaccinate the test agar medium: There are two sorts of immunization that should be possible.

Spot Inoculation

Touch a province of the creature under test with a circle and immunize it onto a little territory of the DNase test agar plate, amidst one of the stamped areas to shape a thick plaque of development 5-10mm in distance across after brooding.

Brood the plate at 37°C for 18-24hr.

Test results
1. DNase Test positive organisms:
1. Serratia marcescens
2. Staphylococcus aureus
3. Campylobacter jejuni (some strains)
4. M. Catarrhalis
2. DNase test negative organisms:
1. Staphylococcus epidermidis
2. Neisseria gonorrhoeae


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