|
#1
May 29th, 2017, 10:49 AM
| |||
| |||
| IIT Bombay HR
Is there anybody who will tell me about features of High Resolution (HR) Liquid Chromatograph Mass Spectrometer facility available at IIT Bombay? Give list of all Specifications of High Resolution (HR) Liquid Chromatograph Mass Spectrometer available at IIT Bombay?
|
|
#2
May 29th, 2017, 10:52 AM
| |||
| |||
| Re: IIT Bombay HR
AS you want to know about High Resolution (HR) Liquid Chromatograph Mass Spectrometer available at IIT Bombay, so here are following information: High Resolution (HR) Liquid Chromatograph Mass Spectrometer Make : Agilent Technologies, USA Model : 1290 Infinity UHPLC System,1260 infinity Nano HPLC with Chipcube, 6550 iFunnel Q-TOFs Specification : 1) UHPLC & NanoHPLC for small molecules as well as for large molecules 2) Direct Infusion Mass with ESI & APCI (Positive & Negative mode ionization 3) UHPLC PDA Detector -Mass spectrometer 4) NanoHPLC with Chipcube (Microfluidic column) -Mass spectrometer Main Features of the Instrument : Mass Range : 50-3200 amu Resolution : 40000 FWHM High Mass Accuracy (Typically less than 1ppm) High Sensitivity (1 pg. Reserpine S/N 100:1) Ionization Method : API 1) ESI positive & negative 2) APCI positive & negative Direct Infusion for Mass Analysis ( MS , MS /MS) Binary Nano HP- LC system with Mass as detector. UHPLC PDA Mass Spectrometer. User Instructions ; 1. Expected structure or mass can be provided. 2. Solvent for mobile phase should be mentioned or solubility of compound in volatile solvent 3. As HP-LC is available in most departments, LC of submitted samples indicating the operation should be supplied along with the sample. 4. At present C8,C18 & others like cyano, amino, Si columns are available. You can submit the interest for separation or repeatability of HP-LC run. 5. For HPLC PDA analysis, wavelength should be given. 6. Protein samples should be properly digested and desalted. 7 Source of the compound should be mentioned. 8. MS-DS (Material Safety Data Sheet) should be given along with samples to ensure that there sample being given. Samples should not be toxic or hazardous. Samples will not be accepted unless MS-DS. Applications: Qualitative Analysis & Quantitative Analysis Impurity Profiling Metabolite Identification Pharmacokinetics Pharma & biomedical applications Proteomics HR-LCMS Charges includes Service Tax: MPP Software 2876/- 576/- 1295/- Per Sample For small molecules : Industry University National Lab/R&D's Only Mass 6900/- 1295/- 3106/- Per Sample Mass+ compound formula & 9200/- 1726/- 4140/- Per Sample database search LCMS with database (Plant extract, Impurity Profiling and Metabolite Identification) 13800/- 2876/- 6326/- Per Sample For large molecules like proteins : Industry University National Lab/R&D's Protein Identification (Per hour) 13800/- 2876/- 6326/- Per hour Sample Preparation 6900/- 1295/- 3106/- Per Sample Offgel 3100 fractionator 6900/- 1295/- 3106/- Per Sample Contacts Contact : 022-25764667 Email Id : pradnyaj@iitb.ac.in |
|
#3
May 29th, 2017, 10:52 AM
| |||
| |||
| Re: IIT Bombay HR
AS you want to know about High Resolution (HR) Liquid Chromatograph Mass Spectrometer available at IIT Bombay, so here are following information: High Resolution (HR) Liquid Chromatograph Mass Spectrometer Make : Agilent Technologies, USA Model : 1290 Infinity UHPLC System,1260 infinity Nano HPLC with Chipcube, 6550 iFunnel Q-TOFs Specification : 1) UHPLC & NanoHPLC for small molecules as well as for large molecules 2) Direct Infusion Mass with ESI & APCI (Positive & Negative mode ionization 3) UHPLC PDA Detector -Mass spectrometer 4) NanoHPLC with Chipcube (Microfluidic column) -Mass spectrometer Main Features of the Instrument : Mass Range : 50-3200 amu Resolution : 40000 FWHM High Mass Accuracy (Typically less than 1ppm) High Sensitivity (1 pg. Reserpine S/N 100:1) Ionization Method : API 1) ESI positive & negative 2) APCI positive & negative Direct Infusion for Mass Analysis ( MS , MS /MS) Binary Nano HP- LC system with Mass as detector. UHPLC PDA Mass Spectrometer. User Instructions ; 1. Expected structure or mass can be provided. 2. Solvent for mobile phase should be mentioned or solubility of compound in volatile solvent 3. As HP-LC is available in most departments, LC of submitted samples indicating the operation should be supplied along with the sample. 4. At present C8,C18 & others like cyano, amino, Si columns are available. You can submit the interest for separation or repeatability of HP-LC run. 5. For HPLC PDA analysis, wavelength should be given. 6. Protein samples should be properly digested and desalted. 7 Source of the compound should be mentioned. 8. MS-DS (Material Safety Data Sheet) should be given along with samples to ensure that there sample being given. Samples should not be toxic or hazardous. Samples will not be accepted unless MS-DS. Applications: Qualitative Analysis & Quantitative Analysis Impurity Profiling Metabolite Identification Pharmacokinetics Pharma & biomedical applications Proteomics HR-LCMS Charges includes Service Tax: MPP Software 2876/- 576/- 1295/- Per Sample For small molecules : Industry University National Lab/R&D's Only Mass 6900/- 1295/- 3106/- Per Sample Mass+ compound formula & 9200/- 1726/- 4140/- Per Sample database search LCMS with database (Plant extract, Impurity Profiling and Metabolite Identification) 13800/- 2876/- 6326/- Per Sample For large molecules like proteins : Industry University National Lab/R&D's Protein Identification (Per hour) 13800/- 2876/- 6326/- Per hour Sample Preparation 6900/- 1295/- 3106/- Per Sample Offgel 3100 fractionator 6900/- 1295/- 3106/- Per Sample Contacts Contact : 022-25764667 Email Id : pradnyaj@iitb.ac.in |
| |