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June 13th, 2016, 12:39 PM
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Join Date: Mar 2012
Re: Krishna University M.Sc Biotechnology Syllabus

Krishna University offers M.Sc (Biotechnology). The duration of the course is 2 years with four semesters. The course will be conducted on credit system

Krishna University M.Sc (Biotechnology) syllabus


Duration - 2 years

Eligibility-

Students must have clear graduation degree/ 10+2+3 level in life sciences subjects

Entrance test-

The Entrance Test will have 100 objectives multiple choice type questions

The question paper generally will consist of three sections viz., Sections A & B with 30 question each and Section C with 40 questions

Syllabus-

Section-A

Physical Sciences and Mathematics --Atomic number and atomic weight. Electronic
configurations, Electronic theory of valency. Oxidation states, Oxidation numbers. Common redox and
ionic reactions. Nuclear chemistry, composition of nucleus, binding energy and packing fraction, Nuclear
reaction. Uses of radioisotopes, Electron displacements, bond formation and fission and reaction
intermediates, Nucleophiles and electrophiles. Alkanes, alkenes and alkynes, alkyl halides, alcohols,
aldehydes, ketones, acids, esters and amino preparations, properties and reactions, Benzene-aromaticity.
Dilute solutions - Osmotic pressure, lowering of vapour pressure, depression of freezing point and
elevation of boiling point. Molecular weight determination in solution, association and dissociation of
solutes. Chemical kinetics-Molecularity and order of a reaction - first order, second order and rate
equation. Electrochemistry, laws of electrolytes, conductivity of an electrolyte, hydrolysis of salts,
hydrogen ion concentration, buffer solutions and acid-base indicators. Role of metals in biological
systems, sodium, potassium, iron, zinc and cobalt, toxic metals. Arsenic, mercury, lead, inorganic and
organic pollutants - their reactivity and effect of some pollutants on human and plant life.Application of
mathematics in biology. General introduction to computers.

Section-B

Life Sciences: Cell structure and function, Cell organelles, Cell division. Genetics, laws of
inheritance, Linkage, Crossing over, Polyploidy, Hybrid vigour and Sex determination. Structure and
replication of DNA, genetic recombination, transcription and post transcriptional modifications, Concept of
genetic code, RNA and ribosomes - their function and role in protein synthesis. Gene cloning, application
of gene cloning in medicine, Gene Mapping, Mutations. Cell and its biochemical organization - Structure,
chemistry, properties and metabolism of carbohydrates, proteins, fats and vitamins Concept of basic
metabolism, Role of enzymes in Metabolism, Enzyme kinetics, inhibition, and Regulation. Microbiology -
Classification and identification of bacteria, viruses and other microorganisms, structure and isolation,
culture methods, role of microbes in carbon, nitrogen and sulphur cycles in nature. Immunology -
Organism immunity, immunity to infectious diseases and vaccines.

Section C

Principles of Biotechnology:
Principles and applications of centrifugation, Paper and Thin layer chromatography, paper
electrophoresis, agarose gel electrophoresis and their applications. Principle and applications of UVVisible
spectroscopy. Microscopic techniques–Light and electron microscopy. Fermentation - Introduction,
types of fermentation processes (batch, fed-batch, continuous), types of fermenters (Stirred tank & Air-Lift
Fermenters), feed culture preparation, inoculum preparation and product yield. Product Recovery
Techniques (Biomass separation, filtration, centrifugation, membrane separation), Product purification
techniques (Dialysis, reverse osmosis, organic solvent extraction). Production of alcoholic beverages
(Beer and wine), antibiotics (Penicillin), organic acids (Citric acid), amino acids (Glutamic acid). Single cell
proteins and Probiotics. Media used for plant tissue culture, different components of media, sterilization of
media, Callus culture, Meristem culture, Somatic embryogenesis, Synthetic seeds, transgenic plants and
applications. Serum and serum free media used for maintenance of animal cell lines, Sterilization of
media, Maintenance of primary cell line and secondary cell lines, transgenic animals and their
applications. Definition and scope of environmental biotechnology, Municipal solid waste disposal,
Microbiological treatment of municipal sewage and industrial effluents, Waste water treatment system
(Aerobic, anaerobic and activated sludge process), Bioremediation, Biodegradation.


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