Guys, can you provide me the syllabus of Mumbai University M.Sc Microbiology????

The Mumbai University offers M.Sc Microbiology through various affiliated colleges. Here are the details of this course:

Eligibility:
The applicant should have completed graduation in the respective subjects with good academic record from any recognized university of India.

Admission:
For admission in this course you need to appear in the entrance test…………

Syllabus:

Unit I Research Fundamentals and Terminology (15L)
1.1 Meaning and Objective of research, features of a good research study, scientific
method, (05L)
1.2 Study designs and variations: basic, applied, historical, exploratory,
experimental, ex-post-facto, case study, diagnostic research, crossover design,
case control design, cohort study design, multifactorial design (10L)
Unit II Defining Research problem and data Collection (15L)
2.1 Hypothesis, theory and scientific law: development, structure, conditions,
sources, formulation, explanation of hypothesis; structure, identification,
elements, classification, functions of theory; scientific laws and principles (05L)
2.2 Methods and techniques of data collection: types of data, methods of primary
data collection( observation/ experimentation/ questionnaire/ interviewing/ case/
pilot study , methods), methods of secondary data collection( internal/ external),

M.Sc. (Semester -III)Microbiology Syllabus
Revised for Credit Based and Grading System
To be implemented from the Academic year 2013-2014
Semester -III Detail Syllabus
PSMB301: Tools and Techniques: Research Methodology (60L)
Course Code Title
Cred
Its

PSMB301 Tools and Techniques : Research Methodology (60L) 04
Unit I Research Fundamentals and Terminology (15L)
1.1 Meaning and Objective of research, features of a good research study, scientific
method, (05L)

1.2 Study designs and variations: basic, applied, historical, exploratory,
experimental, ex-post-facto, case study, diagnostic research, crossover design,
case control design, cohort study design, multifactorial design (10L)

Unit II Defining Research problem and data Collection (15L)
2.1 Hypothesis, theory and scientific law: development, structure, conditions,
sources, formulation, explanation of hypothesis; structure, identification,
elements, classification, functions of theory; scientific laws and principles (05L)
2.2 Methods and techniques of data collection: types of data, methods of primary
data collection( observation/ experimentation/ questionnaire/ interviewing/ case/
pilot study , methods), methods of secondary data collection( internal/ external),

schedule method (10L)
Unit III Sampling and sampling distributions (15L)
3.1 Sampling frame, importance of probability sampling, simple random sampling,
systematic sampling, stratified random sampling, cluster sampling, problems
due to unintended sampling, ecological and statistical population in the
laboratory, (10L)
3.2 Variables: nominal, ordinal, discontinuous, continuous, derived (05)

Unit IV Data analysis and report writing (15L)
4.1 Experimental data collection and data processing: Processing operations,
problems in processing, elements of analysis in data processing, software for
data processing, (05L)
4.2 Report writing and presentation: types of research reports, guidelines for
writing a report, report format, appendices, Miscellaneous information, poster
and oral presentations (10L)

Practical: PSMB3P1
PSMB3P1
Tools and Techniques: Research Methodology (60L)
Unit I : Literature survey (15L)
Unit II : Literature survey (15L)
Unit III : Writing Research Project Proposal (15L)
Unit IV : Writing Research Project Proposal (15L)
02

References: PSMB301 (Semester III)
1. Kothari, C.R.,1985, Research Methodology- Methods and Techniques, New Delhi,
Wiley Eastern Limited.
2. Das, S.K. , 1986, An Introduction to Research, Kolkata, Mukherjee and Company
Pvt. Ltd.
3. Misra R.P., 1989, Research Methodology: A Handbook, New Delhi, Concept
Publishing Company
4. Kumar, R., 2005, Research Methodology-A Step-by-Step Guide for
for Beginners,(2nd.ed.),Singapore, Pearson Education.
5. Bhattachraya, D.K., 2006, Research Methodology,(2nd.ed.),New Delhi, Excel Books.
6. Panneerselvam R.,2012, Research Methodology, New Delhi, PHI Learning Pvt. Ltd.
7. Khan, Irfan Ali, 2008, Fundamentals of Biostatistics, Ukaaz Publications
8. Rosner B.A., 2011, Fundamentals of Biostatistics, Cengage Learning
9. Katz J.M., 2009, Form Research to Manuscript: A guide to scientific writing, USA,
Springer Science
10. Saravanavel, P. 1990. Research methodology. Allahabad, Kitab Mahal

PSMB302: Food Microbiology
Course Code Title Credits
PSMB302 Food Microbiology (60L) 04
Unit I: Microbes in foods (15L)
1.1 Importance of microbes in food
1.2 Sources of microbes in food
1.3 Normal microbiological quality of food
1.4 Factors influencing microbial growth in food
01

Unit II: Uses of microbes in food (15L)
2.1 Microbial stress response in food
2.2 Starter cultures
2.3 Microbiology of fermented foods General method of
production
2.3.a. Cheese – Swiss and Blue cheese
2.3.b.Fermented meat product – Sausage
2.3.c.Fermented vegetable products – Pickles, soy product ,
Sauerkraut
2.3.d.Bread and Idli

01
Unit : III Control of microbes in food (15L)
3.1 Control of access
3.2 Control by physical removal, heat, low temperature, reduced
aw, low pH and organic acids, modified atmosphere,
antimicrobial preservatives, irradiation
3.3 Novel emerging techniques of food preservation
3.4 Control by combination of methods ( Hurdle concept )

Unit : IV Microbial Detection and Food Safety (15L)
4.1Conventional Methods.
4.1.a. Methods used, Sampling for microbial analysis
4.1.b.Quantitative microbial enumeration in food
4.1.c.Qualitative methods of microbial detection
4.1.d.Bacterial Toxins
4.1.e.Rapid methods
4.1.f.Biosensors
4.2 Controlling the Microbiological Quality of food.
4.2.a. Quality and Criteria
4.2.b.Sampling Schemes
4.2.c.QC using microbiological control
4.2.d.Control at source
4.2.e.Codes of GMP

4.2.f.HACCP
4.2.g.Laboratory Accreditation

Practicals : PSMB3P2:
PSMB3P2
Food Microbiology (60L)
1.Microbiological study of fermented foods ( Idli batter and
sauerkraut )
2.Microbiological load in carrot and apple juice, salad, mayonese.
3.Quality Assessment and Analysis of food
i) Milk ( Raw, Packed )
ii) Ice-cream
iii)Yoghurt
4. Report to be written in journal on Novel detection methods for
food borne pathogens/ toxins.
02

References:PSMB302 (Semester III)
References: Unit I
1. Bibek Ray and Arun Bhunia ( 2008) Fundamental Food
Microbiology 4th Ed. CRC Press.
2. Srilakshami B ( 2010 ) Food Science. 5th Ed. New Age International
Publishers.
3. James Jay , M Loessner and D Golden ( 2005 ) Modern Food
Microbiology 7th Ed.
4. Adams M R and Moss M O ( 2008 ) Food Microbiology 3rd Ed. RSC
Publishing.
5. J Maud Kordylas ( 1991 ) Processing and Preservation of tropical
and subtropical foods. ELBS Macmillan.

References: Unit II
1. Bibek Ray and Arun Bhunia ( 2008) Fundamental Food
Microbiology 4th Ed. CRC Press.
2. Gerald Reed ( 2004 ) Prescott and Dunn’s Industrial Microbiology
4th Ed. CBS Publishers.
3. J Maud Kordylas ( 1991 ) Processing and Preservation of tropical
and subtropical foods. ELBS Macmillan.

References: Unit III
1. Bibek Ray and Arun Bhunia ( 2008) Fundamental Food
Microbiology 4th Ed. CRC Press.
2. N Shakuntala Manay and Shadaksharaswamy M ( 1985 ) Foods
Facts and Principles. New Age International

References: Unit IV
1. Bibek Ray and Arun Bhunia ( 2008) Fundamental Food
Microbiology 4th Ed. CRC Press.
2. Adams M R and Moss M O ( 2008 ) Food Microbiology 3rd Ed. RSC
Publishing.
3. N Shakuntala Manay and Shadaksharaswamy M ( 1985 ) Foods
Facts and Principles. New Age International.
4. Harrigan W F and McCance M F ( 1976 ) Laboratory methods in
food and dairy microbiology. Academic Press.
5. Aylward F ( 2001 ) Food Technology Processing and Laboratory
Control. Agrobios ( India )

PSMB303 : Advances in Biotechnology
Course Code Title Credits
PSMB303 Advances in Biotechnology (60L) 04
Unit I Plant and Agricultural Biotechnology (15L)
1.1 Plant Tissue Culture for crop improvement--Initiation and maintenance of Callus
and Suspension culture, Direct and Indirect Organogenesis, Micropropagation,
Artificial seeds, Anther culture and dihaploids, Protoplast isolation culture and
fusion, Production of haploids, Somaclonal variations, Germplasm conservation,
Somatic hybrids, Cybrids.
1.2 Production of secondary metabolites from plant cell cultures, Technology of plant
cell culture for production of chemicals, Bioreactor systems and models for mass
cultivation of plant cells.
1.3 Plant Transformation Technology – Agrobacterium mediated gene transfer,

Agrobacterium based vectors, viral vectors, Direct gene transfer methods,
chemical methods, electroporation, microinjection, particle bombardment,
Molecular breeding, plant selectable markers, Reporter genes, Positive selection,
Selectable marker elimination, Trangene silencing, Strategies to avoid trangene
silencing.
1.4 Plant Genetic Engineering for Productivity and Performance—
a) Biotic Stress Tolerance- Herbicide resistance, Glyphosate, Insect Resistance, Bt
toxin, Disease Resistance, Virus resistance
b) Abiotic Stress Tolerance-- Drought, Flooding, Salt and temperature.
c) By manipulation of—Photosynthesis, Nitrogen fixation, Nutrient uptake
efficiency
d) For Quality Improvement-Protein, Lipids, carbohydrates, vitamins and
minerals.
e) Biosafety concerns of transgenic plants
1.5 Plants as bioreactors.

UNIT II Animal Biotechnology (15L)
2.1 Animal Tissue Culture: Primary culture, Organ culture, Embryo Culture,
Established Cell lines
2.2 Scale up, Cryopreservation, Culture Collections
2.3 Risks and Safety, Bioethics.
2.4 Stem Cell Technology, Cloning techniques Applications.
2.5 Transgenics and knockouts: Transgenic cattle, Transgenic birds, Transgenic fish
2.6 Applications: Transgenic mice: i) Retroviral method ii) DNA microinjection
method iii) Engineered Embryonic Stem cell method
01

Unit III Nanobiotechnology (15L)
3.1 Nanoscale systems,nanoparticles,nanowires,thin films and multilayers; Properties
of nanomaterials.
01
3.2 Synthesis of nanostuctures - physical, chemical and biological,micobiological
methods -
a. Biomolecules as nanostructures.
b. Nanoparticular carrier systems, Micro and Nanofluidics.
c. Applications: Biosensors, drug and gene delivery systems, chip technologies,
nano imaging,Nanomedicine and Cancer diagnostics and treatment.

Unit IV - Medical Biotechnology (15L)
4.1 Genetic Testing of diseases and disorders, Cancer genetics., Immunogenetics; prenatal
diagnosis-chorionic villus sampling, amniocentesis, Pre-implantation
diagnosis., Genetic counselling.
4.2 Gene therapy-concept, vectors, gene targeting and tissue-specific expression, Antisense
Technology
4.3 Introduction to pharmacogenomics, Pharmacogenetics and toxicogenomics
4.4 Social- genetic discrimination: insurance and employment, human cloning,
foeticide, Sex determination
4.5 Tissue Engineering, Methods of Synthesis, Biomolecular Engineering

Practicals: PSMB3P3

Advances in Biotechnology (60 L)
1. Terminology, Laboratory design of Animal tissue culture
laboratory
2. Preparation of complete medium, Sterilization and sterility
checking.
3. Chick embryo fibroblast culture, viable staining
4. Lymphocyte culture, viable staining and heamocytometer count.

5. Preparation of Nanosilver By Wet reduction
Method(Chemical),using Neem Extact(plants) &
Bacteria(Microbiological)
6. Characterisation of Nanosilver by UV spectrometry and
microscopic methods
7. Antimicrobial effect of Ionic silver and Nanosilver prepared by
above methods.
8. Study of Nanosilver coated Gauze/textiles for antimicrobial effect
on different bacteria

References: Unit I :PSMB303 (Semester III)
1. Plant Biotechnology: The genetic manipulation of plants,2005,A.Slater ,N.Scott &
M.Fowler, Oxford Univ Press, Oxford.
2. Introduction to Plant Biotechnology(3rd Edtn), H.S. Chawla
3. Roberta Smith, Plant Tissue Culture: Techniques and Experiments,2nd Edtn,Academic
Press,2000
4. H.K.Das(ed),Textbook of Biotechnology,Wiley India,2004
5. J.H.Hammond, P.Mcgarvey, and V.Yusibov(eds), Plant Biotechnolgy,Springer
Verlag,Heidelberg,2000
6. B.B.Buchanan, W.Gruissen and R.L.Jones(eds), Biochemistry and Molecular Biology of
Plants,American Society of Plant Biology,Rockville,USA,2000.
7. Plant Biotechnology and Agriculture:Prospects for the 21st Century, Arie altman ,Paul
Michael Hasegawa,
8. Plant Biotechnology and Genetics:Principles, Techniques & Applications, Stewart,
C.Neal,June 2008,John Wiley &Sons
References: Unit II
1. Animal Cell Culture by Ian Freshney
2. Basic Cell Culture. Ed.J.M.Davis 2nd.Ed 2007. Oxford press
3. Animal Cell Culture Sudha Gangal
4. Principles of biotechnology and applications-Glick and Pasternack

References: Unit III
1. Nanobiotechnology by David Goodsell. John Wiley
2. Handbook of Nanostructured biomaterials and their applications in nanobiotechnology by
Nalwa HS 2005. American scientific publishers
3. Nanobiotechnology by Niemeyer CM & Mirkin CA 2005 .Wiley Interscience

References: Unit IV
1. Jogdand S. N., Medical Biotechnology, Himalaya Publishing House, Mumbai, (2008)
2. Judit Pongracz, Mary Keen, Medical Biotechnology, Churchill Livingstone, Elsevier
(2009)
3. Pratibha Nallari & V. Venugopal Rao, Medical Biotechnology, Oxford University Press,
India (2010)

Detailed syllabus is attached with the file given below, please click on it:

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