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June 1st, 2016, 04:13 PM
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PGEM T Plasmid
Hii Buddy , I want to Get Some General Information about pGEM ®-T and pGEM® -T Easy Vector Systems , Can you please Give me Same Details ? The pGEM®-T and pGEM®-T Easy Vector Systems are convenient systems for the cloning of PCR products. These polymerases often add a single deoxyadenosine, in a template-independent fashion, to the 3´-ends of the amplified fragments. Vector Feature : The pGEM ®-T and pGEM ® -T Easy Vectors are linearized vectors with a single 3´-terminal thymidine at both ends. The T-overhangs at the insertion site greatly improve the efficiency of ligation of PCR products by preventing recircularization of the vector and providing a compatible overhang for PCR products generated by certain thermostable polymerases (1,2). Product Components and Storage Conditions ; Includes: • 1.2µg pGEM ® -T Vector (50ng/µl) • 12µl Control Insert DNA (4ng/µl) • 100u T4 DNA Ligase • 200µl 2X Rapid Ligation Buffer, T4 DNA Ligase PRODUCT SIZE CAT.# pGEM®-T Vector System II 1.2µg pGEM ® -T Vector (50ng/µl) • 12µl Control Insert DNA (4ng/µl) • 100u T4 DNA Ligase • 200µl 2X Rapid Ligation Buffer, T4 DNA Ligase • 1.2ml JM109 Competent Cells, High Efficiency (6 × 200µl) Storage Conditions: For Cat.# A3610, A1380, store the Competent Cells at –70°C. Store all other components at –20°C. pGEM®-T and pGEM®-T Easy Vector Systems Information Here I m Attaching pdf file Which Having Information about pGEM®-T and pGEM®-T Easy Vector Systems You Can Download free of cost : Last edited by Neelurk; April 23rd, 2020 at 08:51 AM. |
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