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  #1  
June 1st, 2016, 04:13 PM
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Join Date: Mar 2012
PGEM T Plasmid

Hii Buddy , I want to Get Some General Information about pGEM ®-T and pGEM® -T Easy Vector Systems , Can you please Give me Same Details ?

The pGEM®-T and pGEM®-T Easy Vector Systems are convenient systems for the cloning of PCR products.

These polymerases often add a single deoxyadenosine, in a template-independent fashion, to the 3´-ends of the amplified fragments.


Vector Feature :

The pGEM
®-T and pGEM
®
-T Easy Vectors are linearized vectors with a single
3´-terminal thymidine at both ends. The T-overhangs at the insertion site greatly improve the efficiency of ligation of
PCR products by preventing recircularization of the vector and providing a compatible overhang for PCR products
generated by certain thermostable polymerases (1,2).

Product Components and Storage Conditions ;

Includes:
• 1.2µg pGEM
®
-T Vector (50ng/µl)
• 12µl Control Insert DNA (4ng/µl)
• 100u T4 DNA Ligase
• 200µl 2X Rapid Ligation Buffer, T4 DNA Ligase
PRODUCT SIZE CAT.#

pGEM®-T Vector System II

1.2µg pGEM
®
-T Vector (50ng/µl)
• 12µl Control Insert DNA (4ng/µl)
• 100u T4 DNA Ligase
• 200µl 2X Rapid Ligation Buffer, T4 DNA Ligase
• 1.2ml JM109 Competent Cells, High Efficiency (6 × 200µl)

Storage Conditions: For Cat.# A3610, A1380, store the Competent Cells at –70°C. Store all other components at
–20°C.

pGEM®-T and pGEM®-T Easy Vector Systems Information






Here I m Attaching pdf file Which Having Information about pGEM®-T and pGEM®-T Easy Vector Systems You Can Download free of cost :
Attached Files
File Type: pdf pGEM®-T and pGEM®-T Easy Vector Systems Information.pdf (435.0 KB, 216 views)

Last edited by Neelurk; April 23rd, 2020 at 08:51 AM.


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