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The % base pair values of four nucleic acid samples are provided below. Which one of the
following samples has the highest Tm?
(A) A = 31; T = 21; G = 20; C = 28
(B) A = 26; T = 14; G = 34; C = 26
(C) A = 17; T = 19; G = 33; C = 31
Q.1
(D) A = 20; T = 30; G = 25; C = 25

Which one of the following is TRUE regarding organization of human chromosomes? It is
made up of
(A) histones that are acidic proteins.
(B) extra-chromosomal circular DNA.
(C) chromatin that consists of DNA and basic proteins.
(D) non-chromosomal DNA.

The melting point of unsaturated fatty acid
(A) is not related to the number of double bonds.
(B) increases with increase in the number of double bonds.
(C) is higher than that of its corresponding saturated fatty acid.
Q.3
(D) decreases with increase in the number of double bonds.

Match the hormones in Group I with the metabolic processes in Group II
Group I Group II
P. Progesterone 1. Increases gluconeogenesis in liver
Q. Glucagon 2. Implantation of fertilized ovum
R. Insulin 3. Stimulates spermatogenesis process
S. Androgen 4. Stimulates glucose uptake and storage
(A) P-2, Q-1, R-4, S-3 (B) P-3, Q-2, R-1, S-4
Q.4
(C) P-1, Q-4, R-2, S-3 (D) P-1, Q-2, R-4, S-3
The most abundant immunoglobulin in human blood is
Q.5
(A) IgM. (B) IgA. (C) IgD. (D) IgG.

The process of purification and recovery of a product in biotechnology is known as
(A) upstream processing. (B) downstream processing.
Q.6
(C) incubation. (D) formulation.

If the velocity of an enzyme catalyzed reaction is 60% of vmax , then the ratio of substrate
concentration [S] to Michaelis-Menton constant KM is
Q.7
(A) 1 (B) 1.5 (C) 2 (D) 4

In a DNA replication experiment, 1 μg of 15N DNA is allowed to replicate till two generations
with 14N DNA. The amount (in μg) of 14N DNA formed during the second replication process
is
Q.8
BT- / 24 2
A
(A) 1 (B) 2 (C) 3 (D) 4
Transport activities in cell membranes are carried by ____________; whereas fluidity of
membranes is maintained by ____________.
(A) lipids; proteins
(B) proteins; nucleic acids
(C) lipids; nucleic acids
Q.9
(D) proteins; lipids
Nodules of leguminous plants are a good source for the isolation of bacteria capable of
(A) nitrogen fixation. (B) carbon fixation.
Q.10
(C) cellulase production. (D) amylase production.
Which of the following statements regarding techniques and their applications is NOT correct?
(A) Recombinant DNA Technology: cloning genes and expression of proteins.
(B) Enzyme Linked Immuno Sorbent Assay: recognize antigen and antibody interactions.
(C) Polymerase Chain Reaction: amplify specific DNA sequences.
Q.11
(D) Western Blot: detect DNA in given samples.
Addition of casein to solid media and picking up bacterial colonies that form clear zone is
termed as
(A) differential enrichment. (B) streaking.
Q.12
(C) serial dilution. (D) selective enrichment.

IIT JAM Biotechnology exam paper

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