The Tet-On 3G Tetracycline-Inducible Gene Expression Systems are the third generation of the most powerful, versatile, as well as widely cited inducible mammalian expression systems available. The Tet-On 3G system offers a noteworthy improvement over Tet-On and Tet-On Advanced with significantly reduced basal expression and increased sensitivity to doxycycline, a tetracycline analogue.

Features
• Lowest background and highest sensitivity
• Highest fold induction
• 14 kit combinations—click here for available options
• Monitor induced expression using fluorescent proteins and a bidirectional promoter
• Ready-made Tet-On 3G Cell Lines are available to cut your time in half
• Retroviral (Retro-X) and lentiviral (Lenti-X) formats are supplied with complete viral packaging systems
• Tet-On 3G inducible adenovirus system (Adeno-X) is also available
Applications
• Inducible expression in mammalian cells
• Inducible expression in transgenic mice
• Inducible expression in stem cells and hematopoietic cells (EF-1 alpha promoter version)
• Co-inducible expression of two genes of interest (bicistronic version)
• Co-express a fluorescent protein (mCherry and ZsGreen1 versions)