Here I am giving you information about important dates to apply for ARS national eligibility test organized by Agricultural Scientists Recruitment Board

Important dates :
Issue of forms : January 2014
Last date: 28 February 2014
Exam date : 26.03.2014 – 04.04.2014 in Online mode at 23 Centers across India

Now dates for ARS NET exam is over, next exam notification will publish in September month of 2014 year.

Application Fee:
Application Fee for the General candidates Rs.1000/-.
For OBC candidates Rs.500/-.
For SC/ ST/PC Candidates Rs.250/-.

ARS NET Exam Pattern
Subject Maximum Marks Duration
Paper 1 (General) 200 2-1/2
Paper 2(Professional) 200 2-1/2



ARS NET exam syllabus of AGRICULTURAL BIOTECHNOLOGY
01. AGRICULTURAL BIOTECHNOLOGY
Unit 1: Cell Structure and Function
Prokaryotic and eukaryotic cell architecture, Cell wall, plasma membrane, Structure
and function of cell organelles: vacuoles, mitochondria, plastids, golgi apparatus, ER,
peroxisomes, glyoxisomes. Cell division, regulation of cell cycle, Protein secretion and
targeting, Cell division, growth and differentiation.
Unit 2: Biomolecules and Metabolism
Structure and function of carbohydrates, lipids, proteins and nucleic acids,
Synthesis of carbohydrate, glycolysis, HMP, Citric acid cycle and metabolic
regulation, Oxidative phosphorylation and substrate level phosphorylation, Vitamins,
plant and animal hormones. Functional molecules, antioxidants, nutrient precursor,
HSPs, anti-viral compounds.
Unit 3: Enzymology
Enzymes, structure conformation, classification, assay, isolation, purification and
characterization, catariysis specificity, mechanism of action, active site, regulation of
enzyme activity, multienzyme complexes, immobilized enzymes and protein
engineering, immobilized enzymes and their application.
Unit 4: Molecular Genetics
Concept of gene, Prokaryotes as genetic system, Prokaryotic and eukaryotic
chromosomes, methods of gene isolation and identification, Split genes, overlapping
genes and pseudo genes, Organization of prokaryotic and eukaryotic genes and
genomes including operan, exon, intron, enhancer promoter sequences and other
regulatory elements. Mutation – spontaneous, induced and site-directed,
recombination in bacteria, fungi and viruses, transformation, transduction,
conjugation, transposable elements and transposition.
Unit 5: Gene Expression
Expression of genetic information, operon concept, Transcription – mechanism of
transcription in prokaryotes and eukaryotes, transcription unit, regulatory sequences
and enhancers, activators, repressors, co-activators, Co-repressors in prokaryotes
and eukaryotes, inducible genes and promoters, Transcription factors post
transcriptional modification and protein transport, DNA-protein interaction, Genetic
code. Mechanism of translation and its control, post translational modifications.
Unit 6: Molecular Biology Techniques
Isolation and purification of nucleic acids. Nucleic acids hybridization: Southern,
northern and western blotting hybridization. Immune response monoclonal and
polyclonal antibodies and ELISA, DNA sequencing. Construction and screening of
genomic and C-DNA libraries. Gel electrophoretic techniques. Polymerase chain
reactor spectroscopy, rtPCR ultracentrifugation, chromatography, FISH, RIA etc.
Unit 7: Gene Cloning
Restriction enzymes and their uses. Salient features and uses of most commonly
used vectors i.e. plasmids, bacteriophages, phagmids, cosmids, BACs, PACs and
YACs, binary vectors, expression vectors. Gene cloning and sub-cloning strategies,
chromosome walking, genetic transformation, Basis of animal cloning. Biology. Risk
assessment and IPR.
Unit 8: Molecular Biology