The syllabus of Semester III Biochemical Techniques – I - Paper IV B.Sc. Degree Program/Course in Biochemistry offered by University of Madras is as follows:

Course Outline : (Biochemical Techniques)

UNIT I
Laboratory hygiene and safety – storage and handling of carcinogenic, toxic, poisonous, allergic chemicals and radioactive substances. Waste disposal – Disposal of corrosive wastes, toxic chemicals, radioactive substances and clinical specimens. Approaches to biochemical investigations, perfusion of isolated organ, slice techniques, tracer techniques and mutant studies for elucidation of metabolic pathways. (15hrs)

UNIT II
pH, pOH, Henderson – Hesselbalch equation, buffers, pH of body fluids, buffers in body fluids, red blood cells and tissues. Measurement of pH by indicators, zwitter ions, pH dependent ionization of amino acids and proteins. Colliods and their applications, viscosity, surface tension and Donnan membrane equilibrium. (15hrs)

UNIT III
Principles of electrochemical techniques. Measurement of pH by glass electrode and hydrogen electrode. Oxygen electrode – principles, operation of a Clarke electrode, applications of oxygen electrode. (15hrs)


UNIT IV
General principles of chromatography. Principles, operational procedure and applications of Column chromatography, Thin layer chromatography (TLC), Adsorption chromatography, Partition chromatography: Liquid-liquid chromatography, Countercurrent chromatography (CCC). (15hrs)

UNIT V
Principles, operational procedure and applications of Gas-liquid chromatography (GLC) Ion-exchange chromatography Exclusion (permeation) chromatography Affinity chromatography High performance (pressure) liquid chromatography (HPLC). (15hrs)

Books Recommended
Principles and Techniques of Practical Biochemistry (Paperback)
by Keith Wilson (Editor), John Walker (Editor), John M. Walker (Author) " Fifth Edition 2000
Introductory Practical Biochemistry (Hardcover)
by S. K. Sawhney; Randhir Singh (Editor) 2005
Principles of Physical Biochemistry (2nd Edition) by Kensal E van Holde, Curtis Johnson, and Pui Shing Ho (Hardcover - April 16, 2005)
Physical Biochemistry: Applications to Biochemistry and Molecular Biology by David M. Freifelder (Paperback - Aug 15, 1982)
Instrumental Methods of Chemical Analysis by G R Chatwal and S K Anand (Hardcover - Jun 1980)
Elements of Analytical chemistry – R. Gopalan, K. Rangarajan. Second edition, 2000, Sultan Chand and sons.
Modern experimental biochemistry – Rodney Boyer. Third edition, 2001, Benjamin Cummings Publishers.
Medical laboratory technology, A procedure manual for routine diagnostic tests Volume I – Kanai L. Mukherjee, 2005, Tata McGraw-Hill Publishers.