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April 23rd, 2015, 03:15 PM
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BAMU BSc Biotechnology Syllabus
I want to take admission in B.Sc. Biotechnology Course at BAMU (Dr. Babasaheb Ambedkar Marathwada University) so please give me details including Syllabus?
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#2
April 23rd, 2015, 04:22 PM
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Re: BAMU BSc Biotechnology Syllabus
Dr. Babasaheb Ambedkar Marathwada University (BAMU) is located in Aurangabad, Maharashtra, Republic of India. This University was established on August 23, 1958 by Maharashtra state government. Type: Public/Government Chancellor: K. Sankaranarayanan Vice-Chancellor: Dr. Vijay M. Pandharipande Campus: Urban Affiliations: UGC, NAAC, AIU Bachelor of Science in Biotechnology: Category: Biotechnology Degree: B.Sc. Level: UG Duration: 3 Years Eligibility: Candidates must have minimum eligibility for which is an intermediate (10+2) in science. Subjects studied under this degree: Biochemistry Molecular Biology Biophysics Immunology Genetic Engineering Plant Biotechnology Animal Biotechnology Environmental Biotechnology Agricultural Biotechnology Medical Biotechnology Cell Biology Microbiology BAMU B.Sc. Biotech Syllabus: Third Year B. Sc. Biotechnology XXV -REG Regulation of Gene Expression (3 Credits) Unit 1: Basics of Gene Expression Regulatory elements/ factors: Inculcate concepts with suitable examples for; Cis acting elements, Trans-acting factors. Exceptional proteins behaving Cis-acting. Regulation of transposition of Tn3 and Tn9. Modifications of Cis-acting elements to influence and to affect regulation. Influencing or affecting gene expression as a presence / or absence of functional form of protein factor. Concept of Activator, Co-activator, Repressor (with suitable examples). Examples with mechanisms; specific regulator and global regulator. DNA protein interactions, RNA protein Interactions –conditions favoring and affecting these interactions (this is to be dealt with ref to Motifs). Unit 2: Bacterial Gene Expression Concept of Operon, Regulation of gene expression; positive control –the ara operon, negative control –paradigm the lac operon and attenuation mediated control or posttranscriptional regulatory control –the trp operon. Must include structural organization of above operons, functional relevance of genes within, regulatory circuit, modes by which the operon can be regulated other than above mentioned mode. Concept of Catabolite Repression. Examples of non-catabolite sugars and their regulation, catabolite repression in amino acid metabolism –examples at molecular level. Unit 3: Eukaryotic Gene Expression Activators :- gene specific and generalized type of activator. Domains of activators, protein and DNA/ or RNA binding domain. Modification of activator. Enhancer mediated gene expression –examples. Gene expression of metallothionine gene expression. Response elements such as; steroid hormone response elements, metal response elements, Basal Expression response elements. Regulation of gene expression at a step of activation of basal apparatus, Post initiation gene expression – mechanism of relieving roadblock (stuttering of RNA polymerase) with example. Regulation of mRNA molecules involving both nonstop and nonsense mechanisms. Gene regulation with example –post transcriptional –yeast and Drosophila genes, insulators in genomic imprinting –concept and example. References: 1. Biochemistry –Lehninger 2. Principles of Biochemistry –Nelson and Cox 3. Microbial genetics –David Frifelder 4. Molecular Biology –David Frifelder 5. Genes –IX 6. Genes -X 7. Principles of gene manipulations –Old and Primrose 8. Biochemistry –Jeremy M. Berg, John L. Tymoczko, and Lubert Stryer 9. Principles of Gene Manipulations LPE Pearson -Watson 10. Genetics –Strickberger Third Year B. Sc. Biotechnology XXVI -ITB Introduction to Bioinformatics (3 Credits) UNIT 1 The Internet and Biologist: Internet basics, FTP, Gopher, World wide web. The Gen Bank Sequence Database: Introduction, Primary & Secondary databse, Format vs content: computer vs humans, GenBank Flat File dissection, GCG, ACDEB. Structure Databases: Introduction to structures, PDB, MMDB, Structure file formats, Visualizing structural information, Database structure viewers. UNIT 2 Information Retrieval from Biological Databases: Retrieving database entries, Integrated information retrieval: The entrez system, sequence databases beyond NCBI, Medical Databases The NCBI Database: Introduction, SeqIDS, Bioseq: Sequences, Bioseqsets: Collections of sequences, Seq. Annot: Annotating the sequence, Seqdiscr: Describing the sequence Sequence Alignment and Database Searching: Introduction, Evolutionary basis of sequence alignment, Optimal alignment methods, Substitution scores & gap penalties, Statistical significance of alignments, Database similarity searching, FASTA, BLAST, Low complexity regions, Repetitive elements UNIT 3 Multiple Sequence Alignment: Progressive alignment methods, Motifs and patterns, Hocks, MOST, Probe, Presentation methods, Abscript Phylogenetic Analysis: Elements of phylogenetic models, data analysis: Alignment, substitution model building, tree building and tree evaluation, building methods, searching for trees, hooting trees, Evaluating trees and data, phylogenetic software Some simple practical consideration Predictive Methods Using Nucleotide Sequence: Framework, marking repetitive DNA, Database search, Codon bias detection, Detecting function sites in the DM, Integrated gene passing, Finding tRMA genes UNIT 4 Predictive methods Using Protein Sequences: Protein identity based on composition, Propsearch, Physical properties based on sequences, secondary structure and folding classes, Sspread sopma, Specialized structures of features, Tertiary structure Genome Mapping: Different types of maps: physical, genetical, etc. Synteny, Human genome project, Application of genome mapping, Chromosome maps. Submitting DNA Sequences to the Databses: Introduction, Where to submit, What to submit, How to submit on the world wide web, How to submit with sequin. References: 1. Developmental Biology-Gilbert 2. Foundations of Embryology – Patten 3. Cell and Developmental Biotechnology – Raj Narian Desikar 4. Text book of Bryophytes, Pteridophytes , Gymnosperms and Paleobotany - Subramurti 5. Plant Anatomy and Embryology- S.N. Pandey, A. Chadha 6. Teresa K Attwood and David J. Parry-Smith, Introduction to Bioinformatics, Pearson Education Asia, 2001 7. Bexavanis & Francis, Bioinformatics-A practical guide to the analysis of genes and proteins, John Wiley and Sons, 2001 8. Rushidi, Basics of Bioinformatics, CRC Publications, 2001 9. Irfan Khan and Atiya Khanum, Emerging trends in Bioinformatics, Ukaaz Publishers, 2002 10. David M. Hill, Craig Martiz and Barke Mable, Molecular systematics 11. Khan Imtiyaz alam ,Rai University, Hydrabad:- Elementry Bioinformatics 12. N. Gautam Bioinformatics- Databases and algorithm 13. Bioinformatics: A practical guide to the analysis of genes and proteins A.D. Baxevanis and B.F.F. Ouellette (Eds). 2002 John Wiley and Sons. 14. Bioinformatics: Sequence and Genome Analysis by D.W. Mount, 2001, Cold Spring Harbor Laboratory Press. Third Year B. Sc. Biotechnology XXVII - PGE Principles of Genetic Engineering (3 Credits) Unit 1: DNA modifications and DNA cutting Systems safeguarding DNA –in detail. Concept of restriction endonuclease action with reference to DNA modification. DNA cutting enzymes: Type I, Type II, Type IIs and Type III with reference to properties, essential co-factors, mode of action –specificity and limitations of their applications. DNA modifying enzymes with reference to their structure, function, requirements, reaction and applications: Exonucleases, Endonucleases acting on both single strand and double strand, Polymerases, phosphorylating enzymes, Phosphate removing enzymes, Enzyme adding base/s to the end. Nucleic acid Joining Enzymes: RNA ligase, DNA ligases. Unit 2: Vectors Concept and types of vectors for genetic engineering, Review of plasmids and modifying natural plasmids to construct a plasmid vector –example of pBR322 –applications, versatility and limitations, cloning of DNA with gene disruption strategy. The pUC18/ pUC19 vectors –with reference to their desing, potential also as an inducible vector system. Viral vectors: M13 life cycle and use of M13 as vector, M13mp1 and M13mp2 design, potential and limitations. Single strand preparations for sequencing reactions. Study of λ-genome to estimate potential as vector system. Concept of insertion and replacement vectors –Two examples with design and application Vectors with two replicons: Phagemids –concept, example with design and application potential and limitations. Vectors for use in Eukaryotic cells- Vectors for plant cell: Ti-plasmid, viral Cauliflower mosaic virus, Binary vectors. Vectors for animal cell: P elements, SV40. Artificial chromosomes, their limitations and applications. Transcriptional and Translational fusion vectors –with examples at least two Unit 3: Cloning and Sequencing Shotgun cloning; with reference to use of plasmid as vector, mean of cloning foreign piece of DNA (Construction of Chimera), mean to construct genomic library, mean to select recombinant with the use of antibiotic marker –a direct selection example. Generalized strategy of obtaining cDNA from mRNA as a template for PCR (do not teach PCR) or piece of DNA to be cloned in desired vectors. DNA sequencing by chemical method, by Enzymatic method and Chemical synthesis of DNA. References: 1. An introduction to Genetic Engineering –Desmond S T Nicholl ,Cambridge university press, 2nd Ed. 2. Recombinant DNA: A short Course, Watson J.D, CSHL press 3. Short course in Bacterial Genetics –J. H. Miller 4. Molecular Biotechnology Principles & Applications of Recombinant DNA, Bernard R Glick & Jack J Pasternak, ASM press. 5. Old R.W & Primrose S.B., Principles of Gene manipulations, Blackwell Scientific publications. 6. Ausbel S.M , Brent R, Current Protocols in Molecular Biology., Wiley International New York. 7. Maniatis I, Fritchh E.F ,& Sambrook J, Molecular cloning. 8. D.M Glover , DNA cloning, A practical approach. 9. Methods in Enzymology series, vol 152, 185, Academic press inc, Sandiego. 10. Genes V –Benjamin Lewin Contact Details: Dr. Babasaheb Ambedkar Marathwada University Dr B A Marathwada University Compound University Campus, Samarth Nagar, Samarth Nagar Aurangabad, Maharashtra 431001 0240 233 2734 more BAMU B.Sc. Biotech Syllabus detail to atteched pdf file; |
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