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Old April 23rd, 2015, 03:45 PM
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Default BAMU BSc Biotechnology Syllabus

I want to take admission in B.Sc. Biotechnology Course at BAMU (Dr. Babasaheb Ambedkar Marathwada University) so please give me details including Syllabus?
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Old April 23rd, 2015, 04:52 PM
Garima Chauhan
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Join Date: Mar 2012
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Default Re: BAMU BSc Biotechnology Syllabus

Dr. Babasaheb Ambedkar Marathwada University (BAMU) is located in Aurangabad, Maharashtra, Republic of India. This University was established on August 23, 1958 by Maharashtra state government.

Type: Public/Government
Chancellor: K. Sankaranarayanan
Vice-Chancellor: Dr. Vijay M. Pandharipande
Campus: Urban
Affiliations: UGC, NAAC, AIU

Bachelor of Science in Biotechnology:
Category: Biotechnology
Degree: B.Sc.
Level: UG
Duration: 3 Years

Candidates must have minimum eligibility for which is an intermediate (10+2) in science.

Subjects studied under this degree:
Molecular Biology
Genetic Engineering
Plant Biotechnology
Animal Biotechnology
Environmental Biotechnology
Agricultural Biotechnology
Medical Biotechnology
Cell Biology

BAMU B.Sc. Biotech Syllabus:
Third Year B. Sc. Biotechnology
Regulation of Gene Expression (3 Credits)
Unit 1: Basics of Gene Expression
Regulatory elements/ factors: Inculcate concepts with suitable examples for; Cis acting
elements, Trans-acting factors. Exceptional proteins behaving Cis-acting. Regulation of
transposition of Tn3 and Tn9. Modifications of Cis-acting elements to influence and to
affect regulation. Influencing or affecting gene expression as a presence / or absence of
functional form of protein factor. Concept of Activator, Co-activator, Repressor (with
suitable examples). Examples with mechanisms; specific regulator and global regulator.
DNA protein interactions, RNA protein Interactions –conditions favoring and affecting
these interactions (this is to be dealt with ref to Motifs).
Unit 2: Bacterial Gene Expression
Concept of Operon, Regulation of gene expression; positive control –the ara operon,
negative control –paradigm the lac operon and attenuation mediated control or posttranscriptional
regulatory control –the trp operon. Must include structural organization of
above operons, functional relevance of genes within, regulatory circuit, modes by which
the operon can be regulated other than above mentioned mode. Concept of Catabolite
Repression. Examples of non-catabolite sugars and their regulation, catabolite repression
in amino acid metabolism –examples at molecular level.
Unit 3: Eukaryotic Gene Expression
Activators :- gene specific and generalized type of activator. Domains of activators,
protein and DNA/ or RNA binding domain. Modification of activator. Enhancer
mediated gene expression –examples. Gene expression of metallothionine gene
expression. Response elements such as; steroid hormone response elements, metal
response elements, Basal Expression response elements. Regulation of gene expression
at a step of activation of basal apparatus, Post initiation gene expression – mechanism of
relieving roadblock (stuttering of RNA polymerase) with example. Regulation of mRNA
molecules involving both nonstop and nonsense mechanisms. Gene regulation with
example –post transcriptional –yeast and Drosophila genes, insulators in genomic
imprinting –concept and example.
1. Biochemistry –Lehninger
2. Principles of Biochemistry –Nelson and Cox
3. Microbial genetics –David Frifelder
4. Molecular Biology –David Frifelder
5. Genes –IX
6. Genes -X
7. Principles of gene manipulations –Old and Primrose
8. Biochemistry –Jeremy M. Berg, John L. Tymoczko, and Lubert Stryer
9. Principles of Gene Manipulations LPE Pearson -Watson
10. Genetics –Strickberger
Third Year B. Sc. Biotechnology
Introduction to Bioinformatics (3 Credits)
The Internet and Biologist: Internet basics, FTP, Gopher, World wide web.
The Gen Bank Sequence Database: Introduction, Primary & Secondary databse,
Format vs content: computer vs humans, GenBank Flat File dissection, GCG, ACDEB.
Structure Databases: Introduction to structures, PDB, MMDB, Structure file formats,
Visualizing structural information, Database structure viewers.
Information Retrieval from Biological Databases: Retrieving database entries,
Integrated information retrieval: The entrez system, sequence databases beyond NCBI,
Medical Databases
The NCBI Database: Introduction, SeqIDS, Bioseq: Sequences, Bioseqsets: Collections
of sequences, Seq. Annot: Annotating the sequence, Seqdiscr: Describing the sequence
Sequence Alignment and Database Searching: Introduction, Evolutionary basis of
sequence alignment, Optimal alignment methods, Substitution scores & gap penalties,
Statistical significance of alignments, Database similarity searching, FASTA, BLAST,
Low complexity regions, Repetitive elements
Multiple Sequence Alignment: Progressive alignment methods, Motifs and patterns,
Hocks, MOST, Probe, Presentation methods, Abscript
Phylogenetic Analysis: Elements of phylogenetic models, data analysis: Alignment,
substitution model building, tree building and tree evaluation, building methods,
searching for trees, hooting trees, Evaluating trees and data, phylogenetic software
Some simple practical consideration
Predictive Methods Using Nucleotide Sequence: Framework, marking repetitive
DNA, Database search, Codon bias detection, Detecting function sites in the DM,
Integrated gene passing, Finding tRMA genes
Predictive methods Using Protein Sequences: Protein identity based on composition,
Propsearch, Physical properties based on sequences, secondary structure and folding
classes, Sspread sopma, Specialized structures of features, Tertiary structure
Genome Mapping: Different types of maps: physical, genetical, etc. Synteny, Human
genome project, Application of genome mapping, Chromosome maps.
Submitting DNA Sequences to the Databses: Introduction, Where to submit, What to
submit, How to submit on the world wide web, How to submit with sequin.
1. Developmental Biology-Gilbert
2. Foundations of Embryology – Patten
3. Cell and Developmental Biotechnology – Raj Narian Desikar
4. Text book of Bryophytes, Pteridophytes , Gymnosperms and Paleobotany - Subramurti
5. Plant Anatomy and Embryology- S.N. Pandey, A. Chadha
6. Teresa K Attwood and David J. Parry-Smith, Introduction to Bioinformatics, Pearson
Education Asia, 2001
7. Bexavanis & Francis, Bioinformatics-A practical guide to the analysis of genes and
proteins, John Wiley and Sons, 2001
8. Rushidi, Basics of Bioinformatics, CRC Publications, 2001
9. Irfan Khan and Atiya Khanum, Emerging trends in Bioinformatics, Ukaaz Publishers,
10. David M. Hill, Craig Martiz and Barke Mable, Molecular systematics
11. Khan Imtiyaz alam ,Rai University, Hydrabad:- Elementry
12. N. Gautam Bioinformatics- Databases and algorithm
13. Bioinformatics: A practical guide to the analysis of genes and proteins A.D.
Baxevanis and B.F.F. Ouellette (Eds). 2002 John Wiley and Sons.
14. Bioinformatics: Sequence and Genome Analysis by D.W. Mount, 2001, Cold Spring
Harbor Laboratory Press.
Third Year B. Sc. Biotechnology
Principles of Genetic Engineering (3 Credits)
Unit 1: DNA modifications and DNA cutting
Systems safeguarding DNA –in detail. Concept of restriction endonuclease action with
reference to DNA modification.
DNA cutting enzymes: Type I, Type II, Type IIs and Type III with reference to
properties, essential co-factors, mode of action –specificity and limitations of their
DNA modifying enzymes with reference to their structure, function, requirements,
reaction and applications: Exonucleases, Endonucleases acting on both single strand and
double strand, Polymerases, phosphorylating enzymes, Phosphate removing enzymes,
Enzyme adding base/s to the end.
Nucleic acid Joining Enzymes: RNA ligase, DNA ligases.
Unit 2: Vectors
Concept and types of vectors for genetic engineering, Review of plasmids and modifying
natural plasmids to construct a plasmid vector –example of pBR322 –applications,
versatility and limitations, cloning of DNA with gene disruption strategy.
The pUC18/ pUC19 vectors –with reference to their desing, potential also as an inducible
vector system.
Viral vectors: M13 life cycle and use of M13 as vector, M13mp1 and M13mp2 design,
potential and limitations. Single strand preparations for sequencing reactions.
Study of λ-genome to estimate potential as vector system. Concept of insertion and
replacement vectors –Two examples with design and application
Vectors with two replicons: Phagemids –concept, example with design and application
potential and limitations.
Vectors for use in Eukaryotic cells- Vectors for plant cell: Ti-plasmid, viral Cauliflower
mosaic virus, Binary vectors. Vectors for animal cell: P elements, SV40.
Artificial chromosomes, their limitations and applications.
Transcriptional and Translational fusion vectors –with examples at least two
Unit 3: Cloning and Sequencing
Shotgun cloning; with reference to use of plasmid as vector, mean of cloning foreign
piece of DNA (Construction of Chimera), mean to construct genomic library, mean to
select recombinant with the use of antibiotic marker –a direct selection example.
Generalized strategy of obtaining cDNA from mRNA as a template for PCR (do not
teach PCR) or piece of DNA to be cloned in desired vectors.
DNA sequencing by chemical method, by Enzymatic method and Chemical synthesis of
1. An introduction to Genetic Engineering –Desmond S T Nicholl ,Cambridge
university press, 2nd Ed.
2. Recombinant DNA: A short Course, Watson J.D, CSHL press
3. Short course in Bacterial Genetics –J. H. Miller
4. Molecular Biotechnology Principles & Applications of Recombinant DNA,
Bernard R Glick & Jack J Pasternak, ASM press.
5. Old R.W & Primrose S.B., Principles of Gene manipulations, Blackwell
Scientific publications.
6. Ausbel S.M , Brent R, Current Protocols in Molecular Biology., Wiley
International New York.
7. Maniatis I, Fritchh E.F ,& Sambrook J, Molecular cloning.
8. D.M Glover , DNA cloning, A practical approach.
9. Methods in Enzymology series, vol 152, 185, Academic press inc, Sandiego.
10. Genes V –Benjamin Lewin

Contact Details:
Dr. Babasaheb Ambedkar Marathwada University
Dr B A Marathwada University Compound
University Campus, Samarth Nagar, Samarth Nagar
Aurangabad, Maharashtra 431001 ‎
0240 233 2734

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