I am providing you the question paper of Molecular Biology of M.D Bio-Chemistry of Rajiv Gandhi University of Health Sciences

RGUHS M.D Bio-Chemistry Molecular Biology question paper

Main Questions (20marks)

1. Genetic code; hybridoma technology.
2. Discuss in detail the regulation of gene expression and genetic engines.
3. Discuss in detail the regulation of gene activity. Write a note on inhibitors of protein synthesis.
4. Write an essay on metabolism of purine nucleotides, purine salvage pathway and gout.
5. Enumerate salient features of eukaryotic protein biosynthesis. Add a note on its inhibitors.
6. What is meant by recombinant DNA technology. Describe the various steps of preparation of recombinant DNA. What are the various applications of recombinant DNA technology.
7. Describe the principle, method, types and application of PCR.
8. Describe in various steps involved in DNA replication and its regulation. Add a note on the importance of telomerase.
9. Discuss the technique in cloning with its application in medicine.

Short Essay Questions (10 marks)

1. Salvage pathway (R) of purine nucleotide biosynthesis.
2. Synthesis of pyrimidine nucleotides
3. Degradation of purine nucleotides and its disorders
4. Metabolism of purine metabolism and its regulations
5. Synthetic analogues of purine and pyrimidine nucleotides
6. Hyperuricemias
7. GOUT
8. t-RNA
9. micro RNA
10. Structure of DNA
11. Histones
12. Secondary structure of DNA
13. A, B and Z DNA.
14. Repair of DNA, DNA repair mechanism
15. Okazaki fragments
16. Replication of DNA (R)
17. Pribnow (TATA) box
18. Briefly discuss the synthesis of protein including the role of initiation factors
19. Reverse transcriptases, give examples and their significance
20. Post transcriptional processing
21. Oncogenes
22. Mutations (R)
23. Point mutations (R)
24. Chemical mutagenesis
25. Non specific mutageneis
26. Gene therapy(R)
27. Chimeric DNA (R), cDNA.
28. Techniques for introduction of DNA into mammalian cells
29. Role of molecular scissors in recombinant DNA technology (R)
30. Molecular cloning in clinical diagnosis and management
31. Probes used in recombinant DNA technology
32. Expression vectors
33. Vectors used in recombinant DNA technology
34. DNA finger printing and its applications
35. DNA microarrays
36. Polymerase chain reaction; principle and application (Rn) and site directed mutagenesis
37. Restricted fragment length polymorphism.
38. Detection of polymorphism
39. Human genome project (Rn)
40. Genetic code
41. Hypbridoma technology (Rn)
42. Medical genetics
43. Genomic imprinting.

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Rajiv Gandhi University of Health Sciences
4th T Block, Jayanagar, Bengaluru, Karnataka 560041