There are 351 American colleges and universities classified as Division I. Division I is the highest level of intercollegiate athletics sanctioned by the National Collegiate Athletic Association (NCAA) in the United States.

List of these institutions is as follows :
Southeast Missouri State University
Utah State University
Lehigh University
Florida A&M University
Delaware State University
University of Tennessee
University at Buffalo, SUNY
Eastern Kentucky University
University of Delaware
Villanova University
Southern University
University of North Texas
North Carolina Agricultural and Technical State University
Austin Peay State University
University of the Pacific
South Dakota State University
High Point University
University of Texas–Pan American
Drake University
University of California, Riverside
University of North Carolina at Chapel Hill
University of Central Florida
Western Carolina University
University of Wisconsin–Madison
Marist College
Loyola University Chicago
University of Nevada, Las Vegas
Saint Francis University
New Jersey Institute of Technology
University of Louisiana at Monroe
University of Louisiana at Lafayette
Idaho State University
Robert Morris University
College of William & Mary
Western Illinois University
University of Memphis
University of Maryland, College Park
Central Connecticut State University
University of Missouri–Kansas City
Long Island University–Brooklyn
University of Wisconsin–Green Bay
Georgetown University
Miami University
Virginia Commonwealth University
University of Hartford
Saint Mary's College of California
Drexel University
Youngstown State University
Old Dominion University
University of Tulsa
California State University, Northridge
Grambling State University
Mississippi State University
Iona College
Manhattan College
North Carolina State University
University of Akron
Central Michigan University
Campbell University
Northwestern University
University of Nebraska–Lincoln
University of Alabama at Birmingham
University of South Florida
Louisiana State University
University of Tennessee at Martin
Utah Valley University
Southern Illinois University Edwardsville
University of California, Davis
Wofford College
Tennessee Technological University
University of San Diego
Norfolk State University
Kent State University
Morehead State University
Sam Houston State University
University of Portland
Bryant University
University of North Carolina Wilmington
Bowling Green State University
Providence College
George Washington University
Houston Baptist University
Virginia Military Institute
Oklahoma State University–Stillwater
Northern Illinois University
University of California, Irvine
University of Arkansas at Pine Bluff
Southern Illinois University Carbondale
University of North Florida
Towson University
Purdue University
Bradley University
Alabama State University
Florida Atlantic University
Boston University
North Dakota State University
Siena College
Fairfield University
Alcorn State University
Wagner College
University of Idaho
California State University, Fullerton
University of Iowa
Xavier University
St. Bonaventure University
University of Pennsylvania
Niagara University
California Polytechnic State University
Vanderbilt University
Oregon State University
Baylor University
Davidson College
University of South Alabama
Tulane University
Coastal Carolina University
Georgia State University
California State University, Sacramento
Auburn University
Florida Gulf Coast University
Kansas State University
University of Arizona
Lipscomb University
Columbia University
University of Miami
Quinnipiac University
Texas Southern University
University of Toledo
University of Arkansas at Little Rock
University of Nevada, Reno
Murray State University
United States Military Academy (Army)
Jacksonville University
Duke University
University of Illinois at Chicago
West Virginia University
University of Tennessee at Chattanooga
Middle Tennessee State University
Boise State University
Portland State University
Texas A&M University–Corpus Christi
University of Notre Dame
St. John's University
The Citadel
University of Houston
Creighton University
University of North Carolina at Asheville
Louisiana Tech University
Temple University
University of Dayton
Hofstra University
Furman University
Washington State University
University of South Dakota
United States Air Force Academy (Air Force)
Rutgers University
Troy University
University of South Carolina
University of Northern Colorado
Northern Arizona University
Saint Joseph's University
Winthrop University
Northwestern State University
Gardner–Webb University
Wright State University
California State University, Long Beach
University of Rhode Island
Liberty University
San Jose State University
University of North Dakota
Ball State University
Rider University
University of Arkansas
University of Southern California
New Mexico State University
University of Texas at Arlington
Loyola University Maryland
Oakland University
Florida International University
Eastern Illinois University
Seattle University
Mount St. Mary's University
Longwood University
Samford University
Pennsylvania State University
University of Kentucky
University of Mississippi
Boston College
Saint Louis University
Indiana University – Purdue University Indianapolis
Bethune-Cookman University
Princeton University
Southern Methodist University
United States Naval Academy (Navy)
Ohio State University
Gonzaga University
College of Charleston
University at Albany, SUNY
Duquesne University
University of South Carolina Upstate
Fordham University
DePaul University
University of Colorado Boulder
Texas A&M University
Indiana State University
San Diego State University
Prairie View A&M University
Clemson University
University of Connecticut
University of California, Berkeley
Stephen F. Austin State University
Mississippi Valley State University
Radford University
George Mason University
California State University, Fresno
University of California, Santa Barbara
Presbyterian College
Pepperdine University
Syracuse University
Savannah State University
East Carolina University
University of Hawaii at Manoa
University of Washington
La Salle University
Cleveland State University
Appalachian State University
Texas State University
Illinois State University
Arizona State University
University of Maryland, Baltimore County
Hampton University
University of San Francisco
University of Illinois at Urbana–Champaign
James Madison University
Valparaiso University
Arkansas State University
South Carolina State University
University of Texas at San Antonio
University of Vermont
Bucknell University
University of Minnesota
Charleston Southern University
North Carolina Central University
Marquette University
Colorado State University
University of Missouri
East Tennessee State University
University of Cincinnati
Tennessee State University
Howard University
Oral Roberts University
Coppin State University
Montana State University
Sacred Heart University
University of Utah
Jacksonville State University
University of Northern Iowa
Weber State University
University of Louisville
Alabama Agricultural and Mechanical University
Iowa State University
University of Central Arkansas
Santa Clara University
Marshall University
Jackson State University
University of New Hampshire
University of Evansville
Colgate University
Wake Forest University
Dartmouth College
Western Kentucky University
Stanford University
Lafayette College
Brigham Young University
Ohio University
Virginia Tech
Wichita State University
University of Michigan
University of Montana
University of California, Los Angeles
University of New Mexico
University of Texas at El Paso
University of Massachusetts Amherst
Indiana University – Purdue University Fort Wayne
Southeastern Louisiana University
Indiana University
University of Georgia
Chicago State University
University of Virginia
Loyola Marymount University
University of Oregon
McNeese State University
Seton Hall University
College of the Holy Cross
Michigan State University
Harvard University
St. Francis College
University of Wyoming
Southern Utah University
Saint Peter's University
Canisius College
Belmont University
California State University, Bakersfield
Lamar University
University of Richmond
University of Maryland Eastern Shore
Morgan State University
Fairleigh Dickinson University
Brown University
Mercer University
Texas Christian University
Eastern Washington University
Cornell University
Kennesaw State University
University of Oklahoma
University of Florida
Missouri State University
Eastern Michigan University
University of Wisconsin–Milwaukee
University of Alabama
Texas Tech University
Yale University
University of Texas at Austin
Nicholls State University
University of Kansas
Monmouth University
Western Michigan University
University of New Orleans
Northeastern University
University of North Carolina at Charlotte
Stetson University
Georgia Southern University
Elon University
University of Detroit Mercy
Stony Brook University
Georgia Institute of Technology
Florida State University
Rice University
University of Maine
Butler University
University of Southern Mississippi
American University
University of Denver
Binghamton University
University of Pittsburgh
University of North Carolina at Greensboro