1.

HPLC stands for
A. High Pressure Liquid Chromatography
B. High Performance Liquid Chromatography
C. both (a) and (b)
D. Highly Placed Liquid Chromatography
t
2.

The eluent strength is a measure of
A. solvent adsorption energy
B. solvent absorption energy
C. solvent diffusivity
D. solvent mixing index
t
3.

HPLC methods include
A. liquid/liquid (partition) chromatography
B. liquid/solid (adsorption) chromatography
C. ion exchange and size exclusion chromatography
D. all of the above
t
4.

Column efficiency is measured in terms of number of plates which is
A. inversely related to the square of the peak width
B. directly related to the square of the peak width
C. inversely related to the cube root of the peak width
D. directly related to the square of the peak width
t
5.

For a typical adsorbent such as silica gel, the most popular pore diameters are
A. 10 and 50 A°
B. 60 and 100 A°
C. 100 and 150 A°
D. 150 and 200 A°


6.

An isocratic elution in HPLC is one in which the composition of the solvent
A. remains constant
B. changes continuously
C. changes in a series of steps
D. none of these
t
7.

In reversed phase HPLC, there is a
A. non polar solvent/polar column
B. polar solvent/non-polar column
C. non polar solvent/non-polar column
D. any of the above
t
8.

Which of the following statements is true for a refractive index detector in HPLC?
A. It is more sensitive than a UV detector
B. It can only be used for isocratic elutions
C. It does not respond to many solutes
D. none of above
t
9.

A gradient elution in HPLC is one in which the composition of the solvent
A. remains constant
B. is changed continuously or in a series of steps
C. both (a) and (b)
D. none of the above
t

10.

An eluotropic series
A. ranks solvents by their relative abilities to displace solutes from a given absorbent
B. ranks column packing material by their relative abilities to retain solutes on the column
C. is a measure of the solvent adsorption energy
D. none of the above