#1
August 2nd, 2016, 12:23 PM
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HPLC Method Development PDF
Hi I am going to represent a presentation on the HPLC Method Development so can you please provide me a PDF on this so I can have an idea???
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#2
August 2nd, 2016, 01:09 PM
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Re: HPLC Method Development PDF
Hi well the HPLC has the following four critical aspects of method development you can have a look and also I have given you the PDF below you can download it Development and Analysis Rapid Sample Preparation – minimum steps for maximum effectiveness, use updated tools (combination filters) and multi- sample preparation equipment (SPE 96-well plates) Choose best bonded phases for high resolution – selecting from typical C18 and C8 bonded phases or those targeted to special sample types Choose the best column configuration for minimum analysis time with high efficiency and resolution – best column length, internal diameter, particle size Using your HPLC instrument to further reduce analysis time and increase sample throughput – optimizing the HPLC and using new features Resolution is driven by the Bonded Phase Choice Changing selectivity (α) influences resolution the most Bonded phase is the column choice that controls selectivity Bonded Phase Choosing Select the columns known to have long lifetimes at operating mobile phase pH. Choose bonded phases on high purity, low acidity silica for best peak shape. Select a C18 or C8 bonded phase first for good retention and resolution with typical acidic, basic and neutral samples. If sample is expected to be difficult (i.e. very polar, difficult to retain, very basic) then select targeted bonded phases available for these types of samples. |
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