As you have asked about the PGCET entrance exam syllabus for Bio
Technology, check below for the information

Engineering Mathematics

Linear Algebra:

Matrices and determinants, Systems of linear equations, Eigen values and
Eigen vectors.

Calculus:

Limit, continuity and differentiability, Partial derivatives, Maxima and
minima, Sequences and series, Test for convergence, Fourier series.

Differential Equations:

Linear and nonlinear first order ODEs, higher order ODEs with
constant coefficients,

Cauchy’s and Euler’s equations, Laplace transforms, PDE-Laplace, heat and
wave equations.

Probability and Statistics:

Probability and Sampling Theorem, Conditional Probability, Mean, median,
mode and standard deviation, Random variables, Poisson, normal and
binomial distributions, Correlation and regression analysis.

Numerical Methods:

Solution of linear and nonlinear algebraic equations, Integration of
trapezoidal and

Simpson’s rule, Single and multistep methods for differential equations.



Biotechnology

Microbiology:

Prokaryotic and eukaryotic cell structure; Microbial nutrition, growth
and control; Microbial metabolism (aerobic and anaerobic respiration,
photosynthesis); Nitrogen fixation; Chemical basis of mutations and
mutagens; Microbial genetics (plasmids, transformation, transduction,
conjugation); Microbial diversity and characteristic features; Viruses.

Biochemistry:

Biomolecules and their conformation; Weak inter-molecular interactions
in biomacromolecules; Chemical and functional nature of enzymes;
Kinetics of single substrate and bi-substrate enzyme catalyzed
reactions; Bioenergetics; Metabolism (Glycolysis, TCA and Oxidative
phosphorylation); Membrane transport and pumps; Cell cycle and cell
growth control; Cell signaling and signal transduction;

Molecular Biology and Genetics:

Molecular structure of genes and chromosomes; DNA replication and
control; Transcription and its control; Translational processes;
Regulatory controls in prokaryotes and eukaryotes; Mendelian
inheritance; Gene interaction; Complementation; Linkage, recombination
and chromosome mapping; Extra chromosomal inheritance;
Chromosomal variation; Population genetics; Transposable elements,
Molecular basis of genetic diseases and applications.

Process Biotechnology:

Bioprocess technology for the production of cell biomass and
primary/secondary

metabolites, such as baker’s yeast, ethanol, citric acid, amino acids,
exopolysacharides, antibiotics and pigments etc.; Microbial production,
purification and bioprocess application(s) of industrial enzymes;
Production and purification of recombinant proteins on a large scale;
Chromatographic and membrane based bioseparation methods;
Immobilization of enzymes and cells and their application for
bioconversion processes. Aerobic and anaerobic biological processes for
stabilization of solid / liquid wastes; Bioremediation.

Bioprocess Engineering:

Kinetics of microbial growth, substrate utilization and product
formation; Simple structured models; Sterilization of air and media;
Batch, fed-batch and continuous processes; Aeration and agitation; Mass
transfer in bioreactors; Rheology of fermentation fluids; Scale-up concepts;
Design of fermentation media; Various types of microbial and enzyme
reactors; Instrumentation in bioreactors.

Plant and Animal Biotechnology:

Special features and organization of plant cells; Totipotency; Regeneration
of plants; Plant products of industrial importance; Biochemistry of
major metabolic pathways and products;

Autotrophic and heterotrophic growth; Plant growth regulators and elicitors;

Cell suspension culture development: methodology, kinetics of growth and
production formation, nutrient ptimization; Production of secondary
metabolites by plant suspension cultures; Hairy root cultures and their
cultivation. Techniques in raising transgencies.

Characteristics of animal cells:

Metabolism, regulation and nutritional requirements for mass cultivation
of animal cell cultures; Kinetics of cell growth and product formation and
effect of shear force; Product and substrate transport; Micro & macro-
carrier culture; Hybridoma technology; Live stock improvement;
Cloning in animals; Genetic engineering in animal cell culture; Animal cell
preservation.

Immunology:

The origin of immunology; Inherent immunity; Humoral and cell mediated
immunity; Primary and secondary lymphoid organ; Antigen; B and T
cells and Macrophages; Major histocompatibility complex (MHC); Antigen
processing and presentation; Synthesis of antibody and secretion;
Molecular basis of antibody diversity; Polyclonal and monoclonal
antibody; Complement; Antigen-antibody reaction; Regulation of immune
response; Immune tolerance; Hyper sensitivity; Autoimmunity; Graft versus
host reaction.

Recombinant DNA Technology:

Restriction and modification enzymes; Vectors: plasmid, bacteriophage
and other viral vectors, cosmids, Ti plasmid, yeast artificial
chromosome; cDNA and genomic DNA library; Gene isolation; Gene
cloning; Expression of cloned gene; Transposons and gene targeting;
DNA labeling; DNA sequencing; Polymerase chain reactions; DNA
fingerprinting; Southern and northern blotting; In-situ hybridization; RAPD;
RFLP; Sitedirected mutagenesis; Gene transfer technologies; Gene therapy.

Bioinformatics:

Major Bioinformatics resources (NCBI, EBI, ExPASy); Sequence and
structure databases;

Sequence analysis (bimolecular sequence file formats, scoring
matrices, sequence alignment, phylogeny); Genomics and Proteomics
(Large scale genome sequencing strategies; Comparative genomics;
Understanding DNA micro arrays and protein arrays); Molecular
modeling and simulations (basic concepts including concept of force
fields).