the sample question paper for the ARS NET Examination which is required for the next examination which you intend to appear is as given below:

1. Endochitinase can be used as an
a) Antibacterial
b) Antiviral
c) Antifungal
d) Antinematodal

2. The direct DNA uptake by the protoplast is stimulated by the chemical
a) Cellulose
b) Sucrose
c) PEG
d) Mannose

3. Which of the following is an herbicide resistant gene?
a) Bar
b) Hpt
c) Chitinase
d) oxidase

4. Restriction enzymes that cuts straight across the DNA produces
a) Nick
b) Sticky ends
c) Blunt ends
d) None of these

5. Lambda has short single stranded 5’ projection of
a) 8 nucleotide length
b) 10 nucleotide length
c) 12 nucleotide length
d) 14 nucleotide length

6. In Agrobacterium mediated plant transformation which one of the following is used to check the Agrobacterium growth
a) PPT
b) Kanamycin
c) Timentin
d) Pencillin

7. The T-DNA region of all the Ti and Ri plasmids are flanked by direct repeat sequence
a) 15bp
b) 20 bp
c) 25 bp
d) 35 bp

8. During Agrobacterium infections the plant cell begins to synthesise an Arginine derivative called as
a) Hygromycin
b) Opines
c) Acetobenzylpurine
d) Acetosyringone

9. During Agrobacterium transformation the T-DNA is coated by protein, which is the product of gene
a) Vir B
b) Vir C
c) Vir D
d) Vir E

10. Most commonly used strategy to develop transgenic plant against any viral disease is to engineer a
a) Anti sense RNA for viral genome
b) Coat or capsid protein gene
c) Direct DNA transfer to viral gene
d) Liposome Mediated transfer of viral RNA

11. The vir region is organised into six operons viz., vir A, vir B, vir C, vir D, vir E, vir G out of these which four operons are required for virulence
a) A,B,C,D
b) B,C,D,E
c) C,D,E,G
d) A,B,D,G

12. Replication of DNA by rolling circle mechanism give rise to a
a) Blunt end DNA
b) Theta structure
c) Concatamer
d) Single strand DNA with nick

13. . The gene transferred from E.coli to tobacco to increase the level of manitol is
a) Manitol dehydrogenase
b) Manitol carboxylase
c) Manitol oxygenase
d) Manitol hydrolase

14. Relaxed plasmid
a) Have only single copy per cell
b) Cannot be purified
c) Does not exist in bacteria
d) Have multiple copies per cell

15. Which of the following is a green tissue specific promoter?
a) Pea vicilin
b) Arabidopsis small subunit of RUBISCO
c) 35s CaMV
d) Bean phytahemaglutinin

16. In vitro packaging is required for which one of the following vector
a) BAC
b) YAC
c) PAC
d) None of these

17. Cosmids have the Cos site of
a) Lambda
b) Plasmid
c) ML3
d) YAC

18. Thiobacillus thioxidans grows on
a) Irron
b)Sulphur
c) Aluminium
d) Pyrites

19. An example of commercially important primary metabolites is
a) Toxin
b) Alkaloid
c) Amine
d) Alcohol

20. Secondary metabolites like antibiotics are not essential for
a) Log phase growth
b) Exponential growth
c) Both a and b
d) none of these

Answers:

1. c) Antifungal

2. c) PEG

3. a) Bar

4. c) Blunt ends

5. c) 12 nucleotide length

6. c) Timentin

7. c) 25 bp

8. b) Opines

9. d) Vir E

10. b) Coat or capsid protein gene

11. d) A,B,D,G

12. c) Concatamer

13. a) Manitol dehydrogenase

14. d) Have multiple copies per cell

15. b) Arabidopsis small subunit of RUBISCO

16. c) PAC

17. a) Lambda

18. d) Pyrites

19. d) Alcohol

20. c) Both a and b