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October 14th, 2016, 09:03 AM
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Join Date: Mar 2012
Genetic Engineering In IIT Bombay

Hello sir, is there any one can provide me syllabus for Genetic Engineering subject topic In IIT Bombay?

At IIT Bombay M.Tech students are required to do substantial amount of course work to complement their undergraduate or masters level education.

The third semester is devoted mostly to M.Tech projects although there are some courses to be taken during that period
As you want here I’m giving you Genetic Engineering IIT Bombay syllabus:


First class or 60% marks (55% for SC/ST) in:

B.Tech./B.E. in Biomedical, Chemical, Computer Science, Electrical, Electronics, Telecommunications, Instrumentation, Mechanical Engineering, Engineering Physics, Metallurgy and Materials Science. OR

M.Sc. or equivalent in Biochemistry, Biophysics, Biotechnology, Ceramics, Chemistry, Electronics, Ergonomics, Materials Science, Mathematics, Molecular Biology, Physics and Physiology. OR
M. Pharm. OR
B.Pharm., B.Ds, BVSc degree (duration 4 years and more)

Genetic Engineering syllabus:

Concept of recombinant DNA technology and purpose, basic methology, use of plasmids, Type I, II and III restriction modification systems, type II restriction endonucleases, nomenclature and sequence recognition, mcr and rnrr genotypes, linkers, adaptors, blunt end ligation, homopolymeric tailing, Transformation, methods in screening recombinant DNA. Radioactive and non-radioactive methods for labeling DNA: Nick translation, random priming, use of Klenow enzyme,

T4 DNA polymerase, bacterial alkaline phosphatase, polynucleotide kinase, hybridization techniques, northern, Southern and colony hybridization. Restriction maps and mapping techniques.

PCR technology, enzymes in PCR, hot-start, touchdown PCR, primer design, introduction of restriction sites etc. Construction of cDNA libraries in plasmids, hybrid select translation,

RT-PCR and quantitative RT-PCR. Strategies for maximizing gene expression, prokaryote expression vectors; pMal, GST fusion vectors, pET vectors and their applications in expression, quantitation, purification.

Inclusion bodies, approaches to solubilization, Intein based expression and peification vectors. Cloning in M13 mp vectors, application to DNA sequencing, site-directed mutagenesis; PCR-based mutations.

Transcription vectors. Lambda vectors; insertion and replacement vectors, selection and screening recombinant phage, in vitro packaging, genomic libraries and cDNA cloning, application of lgt10, lgt11, lZAP vectors. Cosmid vectors.

Yeast transformation, yeast cloning vectors, specialized vectors such as gap and retrievers, principles and application of dihybrid systems.

Cloning and expression in mammalian cells, methods of selection and screening, application of reporter genes. Basic principles of transcriptomics and proteomics.


W. Old & Primrose; Principles of Gene Manipulation. S.B.University Press, 6th edition,

T. Maniatis, E.F. Fritsch & J. Sambrook; Molecular Cloning: A Laboratory Manual. CSHL,
3rd edition, 2002.

M.A. Innis, D.H. Gelfand, J.J. Sninsky & T.J. White. PCR Protocols. Academic Press, 1990

Here I’m attaching PDF of M.Tech IIT Bombay syllabus:
Attached Files
File Type: pdf M.Tech IIT Bombay syllabus.pdf (262.4 KB, 89 views)

Last edited by Neelurk; March 6th, 2020 at 11:06 AM.
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