As you Asking for the Syllabus of the Genetic Engineering of the B.tech Bio Technology Course of the Pune University the Syllabus is As Follow


UNIT 1
Techniques and tools in genetic engineering, Southern Blotting, PCR-design and
optimization, RTPCR, Automated DNA sequencing method, sequencing strategies and analysis and applications, micro arrays, flow cytometry, Enzymes used in GE: DNA modifying enzymes, restriction enzymes, modifying enzymes, DNA ligase, polymerase for GE

UNIT 2
Cloning vectors, Plasmids, Multiple cloning sites, selection markers, lambda phage, phgemids, cosmids, M13 vectors, vectors for cloning in eukaryotic cells, Expression Vectors, artificial chromosomes (BACs, YACs)

UNIT 3
Gene libraries, Cloning strategies: DNA cloning, cDNA synthesis, genomic DNA libraries, cDNA library, amplification of gene libraries, identifying the products of cDNA clones, isolation, selection of recombinants

UNIT 4
Screening libraries, Screening of recombinant clones, sequencing, and synthesis of gene, different methods of gene isolation, techniques of DNA sequencing, artificial DNA synthesis, PCR cloning

UNIT 5
Gene transfer technologies, Transformation, Transfection, Translocation, Conjugation. Modification of bacteria and viruses: live vaccines, transgenesis and cloning, Animal transgenesis, Application of transgenic animals, transgenic plants and their applications

UNIT 6
Applications of rDNA technology in health and agriculture: Humulin, Hep B, factorVIII, DNA diagnostics, BT cotton, Golden rice etc, DNA markers for improvement of quality and yield of crops. RFLP, RAPD, AFLP, Gene therapy,Human genome project.

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Contact details
Pune University
Address: Ganeshkhind, Pune, Maharashtra 411007
Phone: 020 2569 6064