Sathyabama Institute of Science and Technology B.Sc - Biotechnology SBB1601 Advanced instrumentation Techniques Syllabus

SATHYABAMA INSTITUTE OF SCIENCE AND TECHNOLOGY SCHOOL OF BIO & CHEMICAL ENGINEERING

SBB1601 ADVANCED INSTRUMENTATION TECHNIQUES
L T P Credits Total Marks
4 0 0 3 100

UNIT 1 NMR SPECTROSCOPY 12 Hrs.
NMR spectroscopy: Principle, Instrumentation, Solvents used in NMR, NMR signals in various compounds, Chemical
shift, Spin-Spin coupling, Coupling constant, Nuclear magnetic double resonance, 13C NMR, 1D and 2D NMR, NOESY
and COSY techniques, Applications of NMR spectroscopy.

UNIT 2 MASS SPECTROSCOPY 12 Hrs.
Mass Spectroscopy: Principle, Instrumentation of Mass Spectroscopy, Types of ionization like electron impact,
chemical, field, FAB and MALDI, Analyzers of Quadrupole and Time of Flight, Mass fragmentation and its rules,
Fragmentation of important functional groups like alcohols, amines, carbonyl groups and alkanes, Meta stable ions,
Mc Lafferty rearrangement, Ring rule, Isotopic peaks, Tandem Mass Instruments, Applications of Mass spectroscopy

UNIT 3 CHROMATOGRAPHIC METHODS 12 Hrs.
Chromatography: Principle, Apparatus / Instrumentation, Chromatographic parameters, Factors involved, Endpoint
determination and Applications of the following a) Paper chromatography b) Thin Layer chromatography c) Ion
exchange chromatography d) Column chromatography e) Gas chromatography f) GC-MS g) High Performance Liquid
chromatography h) LC-MS i) High Performance Thin Layer chromatography k) Super critical fluid chromatography l)
Affinity chromatography

UNIT 4 ELECTROPHORETIC TECHNIQUES
12 Hrs.
Electrophoresis: Principle, Instrumentation and applications of the following: a) Paper electrophoresis b) Gel
electrophoresis c) Capillary electrophoresis d) Zone electrophoresis e) Moving boundary electrophoresis

UNIT 5 X-RAY CRYSTALLOGRAPHY 12 Hrs.
X ray Crystallography: Production of X rays, Different X ray methods, Braggs law, Rotating crystal technique, X ray
powder technique, Types of crystals, Interpretation of diffraction patterns and applications of X-ray diffraction.
Max Hours.60

Text / Reference Books:
1. Prakash M and Arora CK. Laboratory instrumentation. Anmol Publications Pvt. Ltd.
2. Keith Wilson and John Walker (2000). Practical Biochemistry. 5th Edition, Cambridge University Press.
3. Practical Biochemistry by Boyer
4. Nelson DL and Cox MM. (2008). Lehninger Principles of Biochemistry, 5thEd., W.H.Freeman and Company.
5. Willey MJ, Sherwood LM &Woolverton C J. (2013). Prescott, Harley and Klein’sMicrobiology. 9thEd., McGraw Hill.
6. Karp G. (2010) Cell and Molecula r Biology: Concepts and Experiments. 6 thedition. JohnWiley & Sons. Inc.
7. De Robertis EDP and De Robertis EMF. (2006). Cell and Molecular Biology. 8thedition.Lipincott Williams and
Wilkins, Philadelphia.

END SEMESTER EXAMINATION QUESTION PAPER PATTERN
Max. Marks: 100 Exam Duration: 3 Hrs.
PART A: 10 questions of 2 marks each - No choice 20 Marks
PART B: 2 questions from each unit of internal choice; each carrying 16 marks 80 Marks